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김정범

Kim, Jeong Beom
Molecular Biomedicine Lab.
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dc.citation.endPage 818 -
dc.citation.number 9 -
dc.citation.startPage 809 -
dc.citation.title EXPERIMENTAL HEMATOLOGY -
dc.citation.volume 38 -
dc.contributor.author Zaehres, Holm -
dc.contributor.author Koegler, Gesine -
dc.contributor.author Arauzo-Bravo, Marcos J. -
dc.contributor.author Bleidissel, Martina -
dc.contributor.author Santourlidis, Simeon -
dc.contributor.author Weinhold, Sandra -
dc.contributor.author Greber, Boris -
dc.contributor.author Kim, Jeong Beom -
dc.contributor.author Buchheiser, Anja -
dc.contributor.author Liedtke, Stefanie -
dc.contributor.author Eilken, Hanna M. -
dc.contributor.author Graffmann, Nina -
dc.contributor.author Zhao, Xiaoyi -
dc.contributor.author Meyer, Johann -
dc.contributor.author Reinhardt, Peter -
dc.contributor.author Burr, Boris -
dc.contributor.author Waclawczyk, Simon -
dc.contributor.author Ortmeier, Claudia -
dc.contributor.author Uhrberg, Markus -
dc.contributor.author Schoeler, Hans R. -
dc.contributor.author Cantz, Tobias -
dc.contributor.author Wernet, Peter -
dc.date.accessioned 2023-12-22T06:44:55Z -
dc.date.available 2023-12-22T06:44:55Z -
dc.date.created 2014-10-10 -
dc.date.issued 2010-09 -
dc.description.abstract Objective: Generation of induced pluripotent stem (iPS) cells from human cord blood (CB)-derived unrestricted somatic stem cells and evaluation of their molecular signature and differentiation potential in comparison to human embryonic stem cells. Materials and Methods: Unrestricted somatic stem cells isolated from human CB were reprogrammed to iPS cells using retroviral expression of the transcription factors OCT4, SOX2, KLF4, and C-MYC. The reprogrammed cells were analyzed morphologically, by quantitative reverse transcription polymerase chain reaction, genome-wide microRNA and methylation profiling, and gene expression microarrays, as well as in their pluripotency potential by in vivo teratoma formation in severe combined immunodeficient mice and in vitro differentiation. Results: CB iPS cells are very similar to human embryonic stem cells morphologically, at their molecular signature, and in their differentiation potential. Conclusions: Human CB-derived unrestricted somatic stem cells offer an attractive source of cells for generation of iPS cells. Our findings open novel perspectives to generate human leukocyte antigen-matched pluripotent stem cell banks based on existing CB banks. Besides the obvious relevance of a second-generation CB iPS cell bank for pharmacological and toxicological testing, its application for autologous or allogenic regenerative cell transplantation appears feasible. -
dc.identifier.bibliographicCitation EXPERIMENTAL HEMATOLOGY, v.38, no.9, pp.809 - 818 -
dc.identifier.doi 10.1016/j.exphem.2010.05.009 -
dc.identifier.issn 0301-472X -
dc.identifier.scopusid 2-s2.0-77955486402 -
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/7085 -
dc.identifier.url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=77955486402 -
dc.identifier.wosid 000281168700011 -
dc.language 영어 -
dc.publisher ELSEVIER SCIENCE INC -
dc.title Induction of pluripotency in human cord blood unrestricted somatic stem cells -
dc.type Article -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -

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