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Do, Yoonkyung
DC-based Immune System & Immunotherapy (DISNI) Lab
Research Interests
  • Study on various subsets of dendritic cells and their immunological functions
  • Vaccine development by targeting pathogenic antigens to distinct DC subsets via anti-DC-subset-specific-receptor monoclonal antibodies
  • Characterization of roles of DCs in tumor microenvironment and tumor metastasis
  • Studies on role of DCs in neuro-related diseases
  • Study DCs in collaboration with Biotechnology or Engineering field

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Activation through cannabinoid receptors 1 and 2 on dendritic cells triggers NF-kappa B-dependent apoptosis: Novel role for endogenous and exogenous cannabinoids in immunoregulation

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dc.contributor.author Do, Yoonkyung ko
dc.contributor.author McKallip, RJ ko
dc.contributor.author Nagarkatti, M ko
dc.contributor.author Nagarkatti, PS ko
dc.date.available 2014-10-10T01:58:19Z -
dc.date.created 2014-10-08 ko
dc.date.issued 2004-08 -
dc.identifier.citation JOURNAL OF IMMUNOLOGY, v.173, no.4, pp.2373 - 2382 ko
dc.identifier.issn 0022-1767 ko
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/7066 -
dc.identifier.uri http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=4043179097 ko
dc.description.abstract The precise role of cannabinoid receptors (CB)1 and CB2, as well as endogenous ligands for these receptors, on immune cells remains unclear. In the current study, we examined the effect of endogenous and exogenous cannabinoids on murine bone marrow-derived dendritic cells (DCs). Addition of Δ 9-tetrahydrocannabinol (THC), a major psychoactive component found in marijuana or anandamide, an endogenous cannabinoid, to DC cultures induced apoptosis in DCs. DCs expressed CB1 and CB2 receptors and the engagement of both receptors was necessary to trigger apoptosis. Treatment with THC induced caspase-2, -8, and -9 activation, cleavage of Bid, decreased mitochondrial membrane potential, and cytochrome c release, suggesting involvement of death-receptor and mitochondrial pathways. DCs from Bid-knockout mice were sensitive to THC-induced apoptosis thereby suggesting that Bid was dispensable. There was no induction of p44/p42 MAPK, p38 MAPK, or stress-activated protein/JNK pathway in THC-treated DCs. However, THC treatment induced phosphorylation of IκB-α, and enhanced the transcription of several apoptotic genes regulated by NF-κB. Moreover, inhibition of NF-κB was able to block THC-induced apoptosis in DCs. Lastly, in vivo treatment of mice with THC caused depletion of splenic DCs. Together, our study demonstrates for the first time that endogenous and exogenous cannabinoids may suppress the immune response through their ability to induce apoptosis in DCs. ko
dc.description.statementofresponsibility close -
dc.language ENG ko
dc.publisher AMER ASSOC IMMUNOLOGISTS ko
dc.subject DELTA(9)-TETRAHYDROCANNABINOL INDUCES APOPTOSIS ko
dc.subject MULTIPLE-SCLEROSIS ko
dc.subject IMMUNE-SYSTEM ko
dc.subject MICE LACKING ko
dc.subject IN-VITRO ko
dc.subject EXPRESSION ko
dc.subject DEATH ko
dc.subject PROTEIN ko
dc.subject ALPHA ko
dc.subject INVOLVEMENT ko
dc.title Activation through cannabinoid receptors 1 and 2 on dendritic cells triggers NF-kappa B-dependent apoptosis: Novel role for endogenous and exogenous cannabinoids in immunoregulation ko
dc.type ARTICLE ko
dc.identifier.scopusid 2-s2.0-4043179097 ko
dc.identifier.wosid 000223208900022 ko
dc.type.rims ART ko
dc.description.wostc 87 *
dc.description.scopustc 89 *
dc.date.tcdate 2015-05-06 *
dc.date.scptcdate 2014-10-08 *
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