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Ryu, Jungki
Bio-inspired Functional Materials Lab (BFML)
Research Interests
  • Biomimetics, artificial photosynthesis, biomimetic catalysis, CO2 utilization

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Self-assembled, photoluminescent peptide hydrogel as a versatile platform for enzyme-based optical biosensors

Cited 28 times inthomson ciCited 21 times inthomson ci
Title
Self-assembled, photoluminescent peptide hydrogel as a versatile platform for enzyme-based optical biosensors
Author
Kim, Jae HongLim, Seong YoonNam, Dong HeonRyu, JungkiKu, Sook HeePark, Chan Beum
Keywords
Enzyme biosensors; Peptide self-assembly; Photoluminescent hydrogel; Quantum dots
Issue Date
2011-01
Publisher
ELSEVIER ADVANCED TECHNOLOGY
Citation
BIOSENSORS & BIOELECTRONICS, v.26, no.5, pp.1860 - 1865
Abstract
A self-assembled peptide hydrogel consisting of Fmoc-diphenylalanine has been employed as a biosensing platform through the encapsulation of enzyme bioreceptors (e.g., glucose oxidase or horseradish peroxidase) and fluorescent reporters (e.g., CdTe and CdSe quantum dots). Enzymes and quantum dots (QDs) were physically immobilized within the hydrogel matrix in situ in a single step by simply mixing aqueous solution containing QDs and enzymes with monomeric peptide (Fmoc-diphenylalanine) solution. By using atomic force microscopy and scanning transmission electron microscopy, we observed that the self-assembled peptide hydrogel had a three-dimensional network of nanofibers (with a diameter of approximately 70-90nm) that physically hybridized with QDs and encapsulated enzyme bioreceptors with a minimal leakage. We successfully applied the peptide hydrogel to the detection of analytes such as glucose and toxic phenolic compounds by using a photoluminescence quenching of the hybridized QDs. The Michaelis-Menten constant (K M) of the photoluminescent peptide hydrogel was found to be 3.12mM (GOx for glucose) and 0.82mM (HRP for hydroquinone), respectively, which were much lower than those of conventional gel materials. These results suggest that the peptide hydrogel is an alternative optical biosensing platform with practical advantages such as simple fabrication via self-assembly, efficient diffusion of target analytes, and high encapsulation efficiencies for fluorescent reporters and bioreceptors.
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DOI
10.1016/j.bios.2010.01.026
ISSN
0956-5663
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ECHE_Journal Papers
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