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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 939 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 931 | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 217 | - |
dc.contributor.author | Han, JK | - |
dc.contributor.author | Lee, Sung Kuk | - |
dc.date.accessioned | 2023-12-22T12:42:21Z | - |
dc.date.available | 2023-12-22T12:42:21Z | - |
dc.date.created | 2014-09-29 | - |
dc.date.issued | 1995-03 | - |
dc.description.abstract | In this report, we investigated the possibility that a product of phosphatidylinositol 4,5-bisphosphate (PIP2) breakdown, inositol 1,4,5-trisphosphate (Ins(1,4,5)P-3), and its downstream effector, Ca2+, is involved in oocyte maturation. Microinjection of monoclonal antibody specifically directed to PIP2 into oocytes prior to progesterone addition inhibited meiotic maturation. In addition, preventing or suppressing the progesterone-induced increase in [Ca2+](i) and maintaining cytosolic free Ca2+ of oocyte at 0.1 or 0.3 mu M by Br-2-BAPTA buffered Ca2+ solutions, completely blocked maturation. However, raising cytosolic Ca2+ concentration of oocyte to 1.0 or 3.0 mu M, the inhibitory Br-2-BAPTA effects on oocyte maturation was greatly diminished. Finally, microinjection of heparin, a potent antagonist of Ins(1,4,5)P-3 receptor, inhibited progesterone-induced oocyte maturation in a manner that is linearly proportional to its cytoplasmic concentration. These results strongly support the hypothesis that PIP2 turnover as well as Ins(1,4,5)P-3-induced Ca2+ release play crucial roles in regulating normal meiotic cell division in Xenopus oocyte. | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.217, no.3, pp.931 - 939 | - |
dc.identifier.doi | 10.1006/bbrc.1995.2860 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.scopusid | 2-s2.0-0029417187 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/6789 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0029417187 | - |
dc.identifier.wosid | A1995TM60200031 | - |
dc.language | 영어 | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Reducing PIP2 hydrolysis, Ins(1,4,5)P-3 receptor availability, or calcium gradients inhibits progesterone-stimulated Xenopus oocyte maturation | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
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