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Lee, Sung Kuk
Synthetic Biology & Metabolic Engineering Laboratory
Research Interests
  • Biofuels production
  • Gene discovery
  • Protein engineering
  • Development of gene expression systems
  • Metabolic pathway regulation
  • Metabolic pathway optimization using functional genomics
  • Synthetic biology
  • Development of biosensors

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A tightly regulated inducible expression system utilizing the fim inversion recombination switch

Cited 36 times inthomson ciCited 34 times inthomson ci
Title
A tightly regulated inducible expression system utilizing the fim inversion recombination switch
Author
Ham, TSLee, Sung KukKeasling, JDArkin, AP
Keywords
araBAD promoter; E. coli; Expression vector; Fim inversion; Genetic switch; GFP expression
Issue Date
2006-05
Publisher
WILEY-BLACKWELL
Citation
BIOTECHNOLOGY AND BIOENGINEERING, v.94, no.1, pp.1 - 4
Abstract
The fim inversion system of Escherichia coli (E. coli) can behave as a unidirectional switch in an efficient manner. We have developed a new expression system for E. coli, comprising the arabinose-inducible fimEgene and the fim invertible DNA segment containing a constitutively active promoter. In this system, the target gene is cloned with the promoter in the OFF orientation, resulting in no transcribed product. When induced by arabinose, the active promoter is switched to the ON orientation via FimE-catalyzed DNA inversion, and the gene is expressed. Our expression system exhibited very tightly controlled basal expression and high induced expression, with simple induction by inexpensive arabinose. These characteristics make our system suitable for large-scale expression or for production of toxic proteins.
URI
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DOI
10.1002/bit.20916
ISSN
0006-3592
Appears in Collections:
ECHE_Journal Papers
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