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이성국

Lee, Sung Kuk
Synthetic Biology & Metabolic Engineering Lab.
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dc.citation.endPage 1551 -
dc.citation.number 6 -
dc.citation.startPage 1547 -
dc.citation.title BIOTECHNOLOGY PROGRESS -
dc.citation.volume 22 -
dc.contributor.author Lee, Sung Kuk -
dc.contributor.author Keasling, Jay D. -
dc.date.accessioned 2023-12-22T09:39:45Z -
dc.date.available 2023-12-22T09:39:45Z -
dc.date.created 2014-09-29 -
dc.date.issued 2006-12 -
dc.description.abstract We have developed an expression system (Salmonella-based pPro system) containing the Salmonella enterica prpBCDE promoter (P-prpB) and prpR encoding the positive transcriptional regulator of this promoter. In this study, the transcriptional efficiency of the pPro expression system was measured by placing the gene encoding the green fluorescent protein (gfp) under the control of PprpB and growing cells containing this construct in minimal medium supplemented with glucose or glycerol as a sole carbon source. In wild-type Escherichia coli (E. coli) BL21, the system exhibited high induced expression as well as high background expression; however, in E. coli JSB, a sbm-ygfDGHI deletion mutant of E. coli BL21(DE3), the system showed low background expression and high induced expression. The system exhibited homogeneous expression at the single-cell level, highly regulatable expression over a wide range of propionate concentrations, and fully induced expression at a low propionate concentration relative to that needed to induce the system in rich, undefined medium. The expression system is comparable to the widely used T7 promoter-driven expression systems in glucose or glycerol minimal medium. -
dc.identifier.bibliographicCitation BIOTECHNOLOGY PROGRESS, v.22, no.6, pp.1547 - 1551 -
dc.identifier.doi 10.1021/bp060193f -
dc.identifier.issn 8756-7938 -
dc.identifier.scopusid 2-s2.0-33845482705 -
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/6752 -
dc.identifier.url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33845482705 -
dc.identifier.wosid 000242427200009 -
dc.language 영어 -
dc.publisher WILEY-BLACKWELL -
dc.title Effect of glucose or glycerol as the sole carbon source on gene expression from the Salmonella prpBCDE promoter in Escherichia coli -
dc.type Article -
dc.description.journalRegisteredClass scopus -

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