dc.citation.conferencePlace |
US |
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dc.citation.conferencePlace |
Seattle, WA |
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dc.citation.endPage |
253 |
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dc.citation.startPage |
251 |
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dc.citation.title |
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 |
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dc.contributor.author |
Amasia, M |
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dc.contributor.author |
Cozzens, M |
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dc.contributor.author |
Madou, Marc |
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dc.date.accessioned |
2023-12-20T02:39:08Z |
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dc.date.available |
2023-12-20T02:39:08Z |
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dc.date.created |
2019-03-26 |
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dc.date.issued |
2011-10-02 |
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dc.description.abstract |
This paper reports a novel application of ice-valving in a centrifugal platform and its utilization for PCR amplification. These ice-valves form as a result of freezing small plugs of fluid in the channels directly above cooled thermoelectric modules. A reversible, vapor-tight seal, strong enough to withstand the sustained high temperatures due to PCR thermocycling can be formed in as little as 5 seconds. In order to demonstrate the effectiveness of these vapor-tight valves, PCR thermocycling was performed on a stationary centrifugal disc (CD), with no appreciable sample fluid loss. |
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dc.identifier.bibliographicCitation |
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, pp.251 - 253 |
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dc.identifier.issn |
0000-0000 |
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dc.identifier.scopusid |
2-s2.0-84883782745 |
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dc.identifier.uri |
https://scholarworks.unist.ac.kr/handle/201301/66847 |
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dc.language |
영어 |
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dc.publisher |
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 |
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dc.title |
Vapor-tight ice-valving in centrifugal microfluidics for PCR applications |
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dc.type |
Conference Paper |
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dc.date.conferenceDate |
2011-10-02 |
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