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DC Field | Value | Language |
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dc.citation.startPage | 130187 | - |
dc.citation.title | BIORESOURCE TECHNOLOGY | - |
dc.citation.volume | 394 | - |
dc.contributor.author | Ryu, Huichang | - |
dc.contributor.author | Nguyen, Chau Ngoc Minh | - |
dc.contributor.author | Lee, Sung Kuk | - |
dc.contributor.author | Park, Sunghoon | - |
dc.date.accessioned | 2023-12-20T16:35:08Z | - |
dc.date.available | 2023-12-20T16:35:08Z | - |
dc.date.created | 2023-12-18 | - |
dc.date.issued | 2024-02 | - |
dc.description.abstract | The discovery of formate dehydrogenase (Me-FDH1) from Methylorubrum extorquens has provided an avenue for sustainable CO2 fixation and utilization. However, the mass production of Me-FDH1 is challenging due to the presence of its unique tungsto-bis-metalopterin guanine dinucleotide (W-bis-MGD) cofactor, limiting its practical applications. In this study, C. necator H16 is proposed as a host for the large-scale production of Me-FDH1, utilizing fructose as a carbon source and its inherent machinery for cofactor synthesis. In a minimal salt medium, C. necator H16 could produce active Me-FDH1, which exhibited a specific activity of 80 to 100 U/mg for CO2 conversion to formate. In fed batch bioreactor experiments, approximately 50 g CDW/L (cell dry weight/L) and 10,000 U/L Me-FDH1 were achieved within 50 h. This study highlights C. necator H16 as the recombinant host for Me-FDH1, paving the way for the future development of efficient mass-production methods for this crucial enzyme. | - |
dc.identifier.bibliographicCitation | BIORESOURCE TECHNOLOGY, v.394, pp.130187 | - |
dc.identifier.doi | 10.1016/j.biortech.2023.130187 | - |
dc.identifier.issn | 0960-8524 | - |
dc.identifier.scopusid | 2-s2.0-85180364693 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/66715 | - |
dc.identifier.wosid | 001137561800001 | - |
dc.language | 영어 | - |
dc.publisher | Elsevier BV | - |
dc.title | Development of Cupriavidus necator H16 as a host for heterologous production of formate dehydrogenase I of Methylorubrum extorquens: Possibilities and limitations | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Agricultural Engineering;Biotechnology & Applied Microbiology;Energy & Fuels | - |
dc.relation.journalResearchArea | Agriculture;Biotechnology & Applied Microbiology;Energy & Fuels | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Methylorubrum extorquens | - |
dc.subject.keywordAuthor | Formate dehydrogenase 1 (Me-FDH1) | - |
dc.subject.keywordAuthor | Cupriavidus necator H16 | - |
dc.subject.keywordAuthor | Enzyme production | - |
dc.subject.keywordAuthor | Fructose | - |
dc.subject.keywordPlus | AM1 | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ENZYMES | - |
dc.subject.keywordPlus | CO2 | - |
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