Full metadata record
DC Field | Value | Language |
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dc.citation.startPage | 1305023 | - |
dc.citation.title | FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY | - |
dc.citation.volume | 11 | - |
dc.contributor.author | Kim, Min Kyeong | - |
dc.contributor.author | Jeong, Wonwoo | - |
dc.contributor.author | Jeon, Seunggyu | - |
dc.contributor.author | Kang, Hyun-Wook | - |
dc.date.accessioned | 2023-12-20T15:35:12Z | - |
dc.date.available | 2023-12-20T15:35:12Z | - |
dc.date.created | 2023-12-14 | - |
dc.date.issued | 2023-11 | - |
dc.description.abstract | The cell spheroid technology, which greatly enhances cell-cell interactions, has gained significant attention in the development of in vitro liver models. However, existing cell spheroid technologies still have limitations in improving hepatocyte-extracellular matrix (ECM) interaction, which have a significant impact on hepatic function. In this study, we have developed a novel bioprinting technology for decellularized ECM (dECM)-incorporated hepatocyte spheroids that could enhance both cell-cell and -ECM interactions simultaneously. To provide a biomimetic environment, a porcine liver dECM-based cell bio-ink was developed, and a spheroid printing process using this bio-ink was established. As a result, we precisely printed the dECM-incorporated hepatocyte spheroids with a diameter of approximately 160-220 mu m using primary mouse hepatocyte (PMHs). The dECM materials were uniformly distributed within the bio-printed spheroids, and even after more than 2 weeks of culture, the spheroids maintained their spherical shape and high viability. The incorporation of dECM also significantly improved the hepatic function of hepatocyte spheroids. Compared to hepatocyte-only spheroids, dECM-incorporated hepatocyte spheroids showed approximately 4.3- and 2.5-fold increased levels of albumin and urea secretion, respectively, and a 2.0-fold increase in CYP enzyme activity. These characteristics were also reflected in the hepatic gene expression levels of ALB, HNF4A, CPS1, and others. Furthermore, the dECM-incorporated hepatocyte spheroids exhibited up to a 1.8-fold enhanced drug responsiveness to representative hepatotoxic drugs such as acetaminophen, celecoxib, and amiodarone. Based on these results, it can be concluded that the dECM-incorporated spheroid printing technology has great potential for the development of highly functional in vitro liver tissue models for drug toxicity assessment. | - |
dc.identifier.bibliographicCitation | FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, v.11, pp.1305023 | - |
dc.identifier.doi | 10.3389/fbioe.2023.1305023 | - |
dc.identifier.issn | 2296-4185 | - |
dc.identifier.scopusid | 2-s2.0-85177873718 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/66685 | - |
dc.identifier.wosid | 001107386100001 | - |
dc.language | 영어 | - |
dc.publisher | FRONTIERS MEDIA SA | - |
dc.title | 3D bioprinting of dECM-incorporated hepatocyte spheroid for simultaneous promotion of cell-cell and -ECM interactions | - |
dc.type | Article | - |
dc.description.isOpenAccess | TRUE | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology; Multidisciplinary Sciences | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology; Science & Technology - Other Topics | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | liver tissue engineering | - |
dc.subject.keywordAuthor | dECM-incorporated hepatocyte spheroid | - |
dc.subject.keywordAuthor | cell-ECM interaction | - |
dc.subject.keywordAuthor | decellularization | - |
dc.subject.keywordAuthor | 3D bioprinting | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | TOXICITY | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | PREDICTION | - |
dc.subject.keywordPlus | HYDROGEL | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | MODEL | - |
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