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강현욱

Kang, Hyun-Wook
3D Biofabrication Lab.
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dc.citation.startPage 1305023 -
dc.citation.title FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY -
dc.citation.volume 11 -
dc.contributor.author Kim, Min Kyeong -
dc.contributor.author Jeong, Wonwoo -
dc.contributor.author Jeon, Seunggyu -
dc.contributor.author Kang, Hyun-Wook -
dc.date.accessioned 2023-12-20T15:35:12Z -
dc.date.available 2023-12-20T15:35:12Z -
dc.date.created 2023-12-14 -
dc.date.issued 2023-11 -
dc.description.abstract The cell spheroid technology, which greatly enhances cell-cell interactions, has gained significant attention in the development of in vitro liver models. However, existing cell spheroid technologies still have limitations in improving hepatocyte-extracellular matrix (ECM) interaction, which have a significant impact on hepatic function. In this study, we have developed a novel bioprinting technology for decellularized ECM (dECM)-incorporated hepatocyte spheroids that could enhance both cell-cell and -ECM interactions simultaneously. To provide a biomimetic environment, a porcine liver dECM-based cell bio-ink was developed, and a spheroid printing process using this bio-ink was established. As a result, we precisely printed the dECM-incorporated hepatocyte spheroids with a diameter of approximately 160-220 mu m using primary mouse hepatocyte (PMHs). The dECM materials were uniformly distributed within the bio-printed spheroids, and even after more than 2 weeks of culture, the spheroids maintained their spherical shape and high viability. The incorporation of dECM also significantly improved the hepatic function of hepatocyte spheroids. Compared to hepatocyte-only spheroids, dECM-incorporated hepatocyte spheroids showed approximately 4.3- and 2.5-fold increased levels of albumin and urea secretion, respectively, and a 2.0-fold increase in CYP enzyme activity. These characteristics were also reflected in the hepatic gene expression levels of ALB, HNF4A, CPS1, and others. Furthermore, the dECM-incorporated hepatocyte spheroids exhibited up to a 1.8-fold enhanced drug responsiveness to representative hepatotoxic drugs such as acetaminophen, celecoxib, and amiodarone. Based on these results, it can be concluded that the dECM-incorporated spheroid printing technology has great potential for the development of highly functional in vitro liver tissue models for drug toxicity assessment. -
dc.identifier.bibliographicCitation FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, v.11, pp.1305023 -
dc.identifier.doi 10.3389/fbioe.2023.1305023 -
dc.identifier.issn 2296-4185 -
dc.identifier.scopusid 2-s2.0-85177873718 -
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/66685 -
dc.identifier.wosid 001107386100001 -
dc.language 영어 -
dc.publisher FRONTIERS MEDIA SA -
dc.title 3D bioprinting of dECM-incorporated hepatocyte spheroid for simultaneous promotion of cell-cell and -ECM interactions -
dc.type Article -
dc.description.isOpenAccess TRUE -
dc.relation.journalWebOfScienceCategory Biotechnology & Applied Microbiology; Multidisciplinary Sciences -
dc.relation.journalResearchArea Biotechnology & Applied Microbiology; Science & Technology - Other Topics -
dc.type.docType Article -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.subject.keywordAuthor liver tissue engineering -
dc.subject.keywordAuthor dECM-incorporated hepatocyte spheroid -
dc.subject.keywordAuthor cell-ECM interaction -
dc.subject.keywordAuthor decellularization -
dc.subject.keywordAuthor 3D bioprinting -
dc.subject.keywordPlus IN-VITRO -
dc.subject.keywordPlus CULTURE -
dc.subject.keywordPlus TOXICITY -
dc.subject.keywordPlus METABOLISM -
dc.subject.keywordPlus PREDICTION -
dc.subject.keywordPlus HYDROGEL -
dc.subject.keywordPlus SYSTEM -
dc.subject.keywordPlus MODEL -

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