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DC Field | Value | Language |
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dc.citation.startPage | 128911 | - |
dc.citation.title | BIORESOURCE TECHNOLOGY | - |
dc.citation.volume | 376 | - |
dc.contributor.author | Islam, Tayyab | - |
dc.contributor.author | Nguyen-Vo, Thuan Phu | - |
dc.contributor.author | Gaur, Vivek Kumar | - |
dc.contributor.author | Lee, Junhak | - |
dc.contributor.author | Park, Sunghoon | - |
dc.date.accessioned | 2023-12-21T12:38:22Z | - |
dc.date.available | 2023-12-21T12:38:22Z | - |
dc.date.created | 2023-08-02 | - |
dc.date.issued | 2023-05 | - |
dc.description.abstract | The production of 1,3-butanediol (1,3-BDO) from glucose was investigated using Escherichia coli as the host organism. A pathway was engineered by overexpressing genes phaA (acetyl-CoA acetyltransferase), phaB (acetoacetyl-CoA reductase), bld (CoA-acylating aldehyde dehydrogenase), and yqhD (alcohol dehydrogenase). The expression levels of these genes were optimized to improve 1,3-BDO production and pathways that compete with 1,3-BDO synthesis were disrupted. Culture conditions were also optimized, including the C: N ratio, aeration, induction time, temperature, and supplementation of amino acids, resulting in a strain that could produce 1,3BDO at 257 mM in 36 h, with a yield of 0.51 mol/mol in a fed-batch bioreactor experiment. To the best of our knowledge, this is the highest titer of 1,3-BDO production ever reported using biological methods, and our findings provide a promising strategy for the development of microbial cell factories for the sustainable synthesis of other acetyl-CoA-derived chemicals. | - |
dc.identifier.bibliographicCitation | BIORESOURCE TECHNOLOGY, v.376, pp.128911 | - |
dc.identifier.doi | 10.1016/j.biortech.2023.128911 | - |
dc.identifier.issn | 0960-8524 | - |
dc.identifier.scopusid | 2-s2.0-85150250862 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/65205 | - |
dc.identifier.wosid | 001027284100001 | - |
dc.language | 영어 | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Metabolic engineering of Escherichia coli for biological production of 1, 3-Butanediol | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels | - |
dc.relation.journalResearchArea | Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Metabolic engineering | - |
dc.subject.keywordAuthor | 3-BDO | - |
dc.subject.keywordAuthor | E | - |
dc.subject.keywordAuthor | coli | - |
dc.subject.keywordAuthor | Culture condition optimization | - |
dc.subject.keywordPlus | (R)-1,3-BUTANEDIOL PRODUCTION | - |
dc.subject.keywordPlus | MICROBIAL-PRODUCTION | - |
dc.subject.keywordPlus | ALCALIGENES-LATUS | - |
dc.subject.keywordPlus | GENES | - |
dc.subject.keywordPlus | POLY(3-HYDROXYBUTYRATE) | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | PATHWAYS | - |
dc.subject.keywordPlus | COA | - |
dc.subject.keywordPlus | 1,3-PROPANEDIOL | - |
dc.subject.keywordPlus | MUTANTS | - |
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