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Kim, Yong Hwan
Enzyme and Protein Engineering Lab.
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dc.citation.startPage 110264 -
dc.citation.volume 168 - Uyen Thu Phan - Byoung Wook Jeon - Kim, Yong Hwan - 2023-12-21T11:49:17Z - 2023-12-21T11:49:17Z - 2023-05-26 - 2023-08 -
dc.description.abstract Methylorubrum extorquens AM1 has the potential to consume C1 feedstock to produce a wide range of biomaterials, from bioplastic to pharmaceutical. However, the synthetic biology tools for engineering M. extorquens AM1 need to be employed for precise control of recombinant enzyme expression. In this study, we presented an approach to improve the expression level of formate dehydrogenase 1 from M. extorquens AM1 (MeFDH1) using an efficient terminator and 5′-untranslated region (5′-UTR) design for enhanced carbon dioxide (CO2) conversion activity of whole-cell biocatalyst. The rrnB terminator significantly increased mRNA levels of MeFDH1 alpha and beta subunits by 8.2-fold and 11-fold, respectively, compared to the T7 terminator. Moreover, enzyme production was 1.6-fold higher with 2.1 mg/wet cell weight (WCW) using rrnB terminator. Homologous 5′-untranslated regions (5′-UTR) determined based on proteomics data and UTR designer also influenced the expression level of MeFDH1. The 5′-UTR of the formaldehyde activating enzyme (fae) was the strongest, with 2.5-fold higher expression than that of the control sequence (T7g-10L). Furthermore, the electrochemical reaction of recombinant strains as whole-cell biocatalysts was investigated for their applicability to CO2 conversion, showing enhanced formate productivity. The recombinant strain containing the 5′-UTR sequence of fae exhibited formate productivity of 5.0 mM/h, 2.3-fold higher than that of the control strain (T7). Overall, this study suggested practical applications for CO2 conversion into bioavailable formate and provided valuable insights for recombinant expression systems in methylotrophic strains. -
dc.identifier.bibliographicCitation ENZYME AND MICROBIAL TECHNOLOGY, v.168, pp.110264 -
dc.identifier.doi 10.1016/j.enzmictec.2023.110264 -
dc.identifier.issn 0141-0229 -
dc.identifier.scopusid 2-s2.0-85162208116 -
dc.identifier.uri -
dc.identifier.wosid 001018743900001 -
dc.language 영어 -
dc.publisher Elsevier BV -
dc.title Microbial engineering of Methylorubrum extorquens AM1 to enhance CO2 conversion into formate -
dc.type Article -
dc.description.isOpenAccess FALSE -
dc.relation.journalWebOfScienceCategory Biotechnology & Applied Microbiology -
dc.relation.journalResearchArea Biotechnology & Applied Microbiology -
dc.type.docType Article -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.subject.keywordAuthor Methylorubrum extorquens AM1 -
dc.subject.keywordAuthor Terminator -
dc.subject.keywordAuthor 5 & PRIME -
dc.subject.keywordAuthor -UTR design -
dc.subject.keywordAuthor Whole-cell biocatalyst -
dc.subject.keywordAuthor Formate dehydrogenase 1 (MeFDH1) -
dc.subject.keywordAuthor CO2 conversion -
dc.subject.keywordPlus CARBON -
dc.subject.keywordPlus DEHYDROGENASE -
dc.subject.keywordPlus MOLYBDENUM -


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