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Cho, Moo Je
Ulsan National Institute of Science and Technology
Research Interests
  • Calcium Signaling
  • Calmodulin
  • Plant Defense Mechanism

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ISOLATION OF 2 SOYBEAN G-BOX BINDING-FACTORS WHICH INTERACT WITH A G-BOX SEQUENCE OF AN AUXIN-RESPONSIVE GENE

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dc.contributor.author HONG, JC ko
dc.contributor.author CHEONG, YH ko
dc.contributor.author NAGAO, RT ko
dc.contributor.author BAHK, JD ko
dc.contributor.author KEY, JL ko
dc.contributor.author Cho, Moo Je ko
dc.date.available 2014-09-23T07:05:13Z -
dc.date.created 2014-09-19 ko
dc.date.issued 1995-08 ko
dc.identifier.citation PLANT JOURNAL, v.8, no.2, pp.199 - 211 ko
dc.identifier.issn 0960-7412 ko
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/6383 -
dc.description.abstract G-box binding factors (GBFs) constitute a family of plant DNA-binding proteins that bind to the G-box motif, a regulatory cis element present in many plant genes with a palindromic DNA motif of CACGTG. Previously TCCACGTGTC, a G-box motif, from an auxin responsive gene GmAux28 has been identified as a sequence-specific protein-binding site. Here the isolation of two soybean cDNA clones, referred to as SGBF-1 and SGBF-2, encoding proteins which bind to the G-box motif is reported. The primary structure of SGBF-1 and SGBF-2 predicts that these proteins contain a basic leucine zipper (bZIP) DNA-binding domain and an N-terminal proline-rich domain. A dramatic difference in the pattern of protein-DNA complex formation was observed when recombinant SGBF-1 and SGBF-2 proteins were analyzed by electrophoretic mobility shift assays (EMSAs). The SGBF-1 binding pattern obtained with the G-box probe resulted in three major retarded bands while the SGBF-2 formed a single complex. This shows that the characteristically diffuse banding pattern of plant nuclear proteins interacting with the G-box is also observed in a binding assay using only one recombinant GBF. EMSAs were performed with a few selected binding sequences to study the effect of flanking nucleotides to the hexanucleotide G-box core motif. The binding specificity of the SGBF proteins resembles that described for type A cauliflower nuclear G-box binding proteins which bind class I G-box elements [((G)/(T))((C)/(A))CACGTG((G)/(T)) ((A)/(C))]. Phylogenetic analysis of 13 GBF-like proteins from various plant species reveals that the SGBF-1 and SGBF-2 proteins belong to different lineages, suggesting that they may have distinct functions in activating transcription. ko
dc.description.statementofresponsibility close -
dc.language 영어 ko
dc.publisher WILEY-BLACKWELL ko
dc.title ISOLATION OF 2 SOYBEAN G-BOX BINDING-FACTORS WHICH INTERACT WITH A G-BOX SEQUENCE OF AN AUXIN-RESPONSIVE GENE ko
dc.type ARTICLE ko
dc.identifier.scopusid 2-s2.0-0029347042 ko
dc.identifier.wosid A1995RP94800004 ko
dc.type.rims ART ko
dc.description.wostc 63 *
dc.description.scopustc 43 *
dc.date.tcdate 2015-05-06 *
dc.date.scptcdate 2014-09-19 *
dc.identifier.doi 10.1046/j.1365-313X.1995.08020199.x ko
dc.identifier.url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0029347042 ko
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