Related Researcher

Author's Photo

Cho, Moo Je
Ulsan National Institute of Science and Technology
Research Interests
  • Calcium Signaling
  • Calmodulin
  • Plant Defense Mechanism


Molecular cloning and functional characterization of a cDNA encoding nucleosome assembly protein 1 (NAP-1) from soybean

DC Field Value Language Yoon, HW ko Kim, MC ko Lee, SY ko Hwang, I ko Bahk, JD ko Hong, JC ko Ishimi, Y ko Cho, Moo Je ko 2014-09-23T07:05:11Z - 2014-09-19 ko 1995-12 ko
dc.identifier.citation MOLECULAR AND GENERAL GENETICS, v.249, no.5, pp.465 - 473 ko
dc.identifier.issn 0026-8925 ko
dc.identifier.uri -
dc.description.abstract NAP-1, a protein first isolated from mammalian cells, can introduce supercoils into relaxed circular DNA in the presence of purified core histones. Based on its in vitro activity, it has been suggested that NAP-1 may be involved in nucleosome assembly in vivo. We isolated a cDNA clone encoding a soybean NAP-I homolog, SNAP-1. The SNAP-1 cDNA contains an open reading frame of 358 amino acid residues with a calculated molecular weight of 41kDa. The deduced amino acid sequence of SNAP-1 shares sequence similarity with yeast NAP-1 (38%) and human hNRP (32%). Notable features of the deduced sequence are two extended acidic regions thought to be involved in histone binding. SNAP-1 expressed in Escherichia coli induces supercoiling in relaxed circular DNA, suggesting that SNAP-I may have nucleosome assembly activity. The specific activity of SNAP-1 is comparable to that of HeLa NAP-1 in an in vitro assay. Western analysis reveals that SNAP-I is expressed in the immature and young tissues that were examined, while mature tissues such as old leaves and roots, show very little or no expression. NAP-1 homologs also appear to be present in other plant species. ko
dc.description.statementofresponsibility close -
dc.language 영어 ko
dc.publisher Springer Verlag ko
dc.title Molecular cloning and functional characterization of a cDNA encoding nucleosome assembly protein 1 (NAP-1) from soybean ko
dc.type ARTICLE ko
dc.identifier.scopusid 2-s2.0-0029563615 ko
dc.identifier.wosid A1995TM31400001 ko
dc.type.rims ART ko
dc.description.wostc 18 *
dc.description.scopustc 18 * 2015-05-06 * 2014-09-19 *
dc.identifier.doi 10.1007/BF00290572 ko
dc.identifier.url ko
Appears in Collections:
BIO_Journal Papers
Files in This Item:
There are no files associated with this item.

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show simple item record


  • mendeley


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.