Characterization of a cDNA encoding cysteine proteinase inhibitor from Chinese cabbage (Brassica campestris L ssp pekinensis) flower buds
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- Characterization of a cDNA encoding cysteine proteinase inhibitor from Chinese cabbage (Brassica campestris L ssp pekinensis) flower buds
- Lim, CO; Lee, SI; Chung, WS; Park, SH; Hwang, I; Cho, Moo Je
- Chinese cabbage (Brassica campestris L spp pekinensis); cDNA; cystatin; inhibitor; gene expression
- Issue Date
- PLANT MOLECULAR BIOLOGY, v.30, no.2, pp.373 - 379
- A cDNA encoding a new phytocystatin isotype named BCPI-1 was isolated from a cDNA library of Chinese cabbage flower buds. The BCPI-1 clone encodes 199 amino acids resulting in a protein much larger than other known phytocystatins. BCPI-1 has an unusually long C-terminus. A BCPI-1 fusion protein expressed in Escherichia coli strongly inhibits the enzymatic activity of papain, a cysteine proteinase. Genomic Southern blot analysis revealed that the BCPI gene is a member of a small multi-gene family in Chinese cabbage. Northern blot analysis showed that it is differentially expressed in the flower bud, leaf and root.
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