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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 230 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 226 | - |
dc.citation.title | MOLECULES AND CELLS | - |
dc.citation.volume | 7 | - |
dc.contributor.author | Kim, HY | - |
dc.contributor.author | Seo, HS | - |
dc.contributor.author | Jeong, JY | - |
dc.contributor.author | Lee, SY | - |
dc.contributor.author | Cho, Moo Je | - |
dc.contributor.author | Bahk, JD | - |
dc.date.accessioned | 2023-12-22T12:37:35Z | - |
dc.date.available | 2023-12-22T12:37:35Z | - |
dc.date.created | 2014-09-22 | - |
dc.date.issued | 1997-04 | - |
dc.description.abstract | To study physiological roles of plant small GTP binding proteins, we isolated a cDNA clone (ORrab2) encoding the rab-related small GTP binding protein from rice (Oryza sativa L. IR-36) by using human cDNA rab2 as a probe. The deduced amino acid sequence of the ORrab2 gene shared all the conserved regions, important for GTPase/GTP binding activities, with those of other small GTP binding proteins. ORrab2 is a 1028 bp long cDNA, encodes a 23.2 kDa protein which shows 85.2% similarity on the amino acid sequence level to the Hrab2 protein, and was used as a probe. Through Southern and Northern blot analyses, we found that ORrab2 is a single copy gene and actively expressed at the stages of cell division and elongation. We investigated GTP binding abilities by a filter assay procedure. Deletion of a binding motif, GDTGVGKS, within an ORrab2 protein showed a significant decrease of GTP binding affinity, suggesting its important role in nucleotide binding. | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, v.7, no.2, pp.226 - 230 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.scopusid | 2-s2.0-0031591037 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/6376 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0031591037 | - |
dc.identifier.wosid | A1997WX19900013 | - |
dc.language | 영어 | - |
dc.publisher | KOREAN SOC MOLECULAR & CELLULAR BIOLOGY | - |
dc.title | Molecular characterization of a rab-related small GTP binding protein cDNA from rice (Oryza sativa L IR-36) | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
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