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Cho, Moo Je
Ulsan National Institute of Science and Technology
Research Interests
  • Calcium Signaling
  • Calmodulin
  • Plant Defense Mechanism

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Two hevein homologs isolated from the seed of Pharbitis nil L. exhibit potent antifungal activity

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dc.contributor.author Koo, JC ko
dc.contributor.author Lee, SY ko
dc.contributor.author Chun, HJ ko
dc.contributor.author Cheong, YH ko
dc.contributor.author Choi, JS ko
dc.contributor.author Kawabata, S ko
dc.contributor.author Miyagi, M ko
dc.contributor.author Tsunasawa, S ko
dc.contributor.author Ha, KS ko
dc.contributor.author Bae, DW ko
dc.contributor.author Han, CD ko
dc.contributor.author Lee, BL ko
dc.contributor.author Cho, Moo Je ko
dc.date.available 2014-09-23T07:05:00Z -
dc.date.created 2014-09-22 ko
dc.date.issued 1998-01 ko
dc.identifier.citation BIOCHIMICA ET BIOPHYSICA ACTA - PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, v.1382, no.1, pp.80 - 90 ko
dc.identifier.issn 0167-4838 ko
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/6371 -
dc.description.abstract Two antifungal peptides (Pn-AMP1 and Pn-AMP2) have been purified to homogeneity from seeds of Pharbitis nil. The amino acid sequences of Pn-AMP1 (41 amino acid0 residues) and Pn-AMP2 (40 amino acid residues) were identical except that Pn-AMP1 has an additional serine residue at the carboxyl-terminus. The molecular masses of Pn-AMP1 and Pn-AMP2 were confirmed as 4299.7 and 4213.2Da, respectively, Both the Pn-AMPs were highly basic (pI 12.02) and had characteristics of cysteine/glycine rich chitin-binding domain. Pn-AMPs exhibited potent antifungal activity against both chitin-containing and non-chitin-containing fungi in the cell wall. Concentrations required for 50% inhibition of fungal growth were ranged from 3 to 26 mu g/ml for Pn-AMP1 and from 0.6 to 75 mu g/ml for Pn-AMP2. The Pn-AMPs penetrated very rapidly into fungal hyphae and localized at septum and hyphal tips of fungi, which caused burst of hyphal tips. Burst of hyphae resulted in disruption of the fungal membrane and leakage of the cytoplasmic materials. To our knowledge, Pn-AMPs are the first hevein-like proteins that show similar fungicidal effects as thionins do. ko
dc.description.statementofresponsibility close -
dc.language 영어 ko
dc.publisher Elsevier BV ko
dc.title Two hevein homologs isolated from the seed of Pharbitis nil L. exhibit potent antifungal activity ko
dc.type ARTICLE ko
dc.identifier.scopusid 2-s2.0-0002377366 ko
dc.identifier.wosid 000072159400011 ko
dc.type.rims ART ko
dc.description.wostc 84 *
dc.description.scopustc 79 *
dc.date.tcdate 2015-05-06 *
dc.date.scptcdate 2014-09-22 *
dc.identifier.doi 10.1016/S0167-4838(97)00148-9 ko
dc.identifier.url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0002377366 ko
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