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Cho, Moo Je
Ulsan National Institute of Science and Technology
Research Interests
  • Calcium Signaling
  • Calmodulin
  • Plant Defense Mechanism

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NDP kinase 2 interacts with two oxidative stress-activated MAPKs to regulate cellular redox state and enhances multiple stress tolerance in transgenic plants

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dc.contributor.author Moon, H ko
dc.contributor.author Lee, B ko
dc.contributor.author Choi, G ko
dc.contributor.author Shin, S ko
dc.contributor.author Prasad, DT ko
dc.contributor.author Lee, O ko
dc.contributor.author Kwak, SS ko
dc.contributor.author Kim, DH ko
dc.contributor.author Nam, J ko
dc.contributor.author Bahk, J ko
dc.contributor.author Hong, JC ko
dc.contributor.author Lee, SY ko
dc.contributor.author Cho, Moo Je ko
dc.contributor.author Lim, CO ko
dc.contributor.author Yun, DJ ko
dc.date.available 2014-09-23T07:01:13Z -
dc.date.created 2014-09-23 ko
dc.date.issued 2003-01 ko
dc.identifier.citation PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.100, no.1, pp.358 - 363 ko
dc.identifier.issn 0027-8424 ko
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/6279 -
dc.description.abstract NDP kinases (NDPKs) are multifunctional proteins that regulate a variety of eukaryotic cellular activities, including cell proliferation, development, and differentiation. However, much less is known about the functional significance of NDPKs in plants. We show here that NDPK is associated with H2O2-mediated mitogen-activated protein kinase signaling in plants. H2O2 stress strongly induces the expression of the NDPK2 gene in Arabidopsis thaliana (AtNDPK2). Proteins from transgenic plants overexpressing AtNDPK2 showed high levels of autophosphorylation and NDPK activity, and they have lower levels of reactive oxygen species (ROS) than wild-type plants. Mutants lacking AtNDPK2 had higher levels of ROS than wild type. H2O2 treatment induced the phosphorylation of two endogenous proteins whose molecular weights suggested they are AtMPK3 and AtMPK6, two H2O2-activated A. thaliana mitogen-activated protein kinases. In the absence of H2O2 treatment, phosphorylation of these proteins was slightly elevated in plants overexpressing AtNDPK2 but markedly decreased in the AtNDPK2 deletion mutant. Yeast two-hybrid and in vitro protein pull-down assays revealed that AtNDPK2 specifically interacts with AtMPK3 and AtMPK6. Furthermore, AtNDPK2 also enhances the myelin basic protein phosphorylation activity of AtMPK3 in vitro. Finally, constitutive overexpression of AtNDPK2 in Arabidopsis plants conferred an enhanced tolerance to multiple environmental stresses that elicit ROS accumulation in situ. Thus, AtNDPK2 appears to play a previously uncharacterized regulatory role in H2O2-mediated MAPK signaling in plants. ko
dc.description.statementofresponsibility open -
dc.language 영어 ko
dc.publisher NATL ACAD SCIENCES ko
dc.title NDP kinase 2 interacts with two oxidative stress-activated MAPKs to regulate cellular redox state and enhances multiple stress tolerance in transgenic plants ko
dc.type ARTICLE ko
dc.identifier.scopusid 2-s2.0-0037422543 ko
dc.identifier.wosid 000180307100064 ko
dc.type.rims ART ko
dc.description.wostc 215 *
dc.description.scopustc 201 *
dc.date.tcdate 2015-05-06 *
dc.date.scptcdate 2014-09-30 *
dc.identifier.doi 10.1073/pnas.252641899 ko
dc.identifier.url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0037422543 ko
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