There are no files associated with this item.
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 74 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 66 | - |
dc.citation.title | ACS Food Science and Technology | - |
dc.citation.volume | 2 | - |
dc.contributor.author | Han, Hee Jeong | - |
dc.contributor.author | Oh, Yu-Ri | - |
dc.contributor.author | Eom, Gyeong Tae | - |
dc.date.accessioned | 2023-12-21T14:40:20Z | - |
dc.date.available | 2023-12-21T14:40:20Z | - |
dc.date.created | 2023-03-06 | - |
dc.date.issued | 2022-01 | - |
dc.description.abstract | Lactobionic acid (LBA) is a specialty aldonic acid that has been broadly employed in various industries, including food, cosmetics, and pharmaceuticals. In this study, we isolated a new excellent LBA-producing bacterium from soil, designated as strain KRICT-1. A phylogenetic analysis revealed that this strain was identified as Enterobacter cloacae. Previously, we successfully produced LBA using Pseudomonas taetrolens, an excellent LBA-producing bacterium. In a flask culture at 30 °C, E. cloacae KRICT-1 showed the same LBA production concentration (209.3 g/L) and yield (100%) from 200 g/L lactose, compared with those obtained from P. taetrolens cultivated at 25 °C, the growth temperature of which showed the highest LBA production ability. E. cloacae KRICT-1 exhibited an approximately 1.08-fold higher LBA productivity (8.37 g/L/h) than that (7.75 g/L/h) of P. taetrolens, indicating that E. cloacae KRICT-1 was a more efficient LBA-producing bacterium than P. taetrolens. The LBA productivity of E. cloacae KRICT-1 further improved to 8.72 g/L/h at 35 °C. Furthermore, the LBA productivity (9.97 g/L/h) improved at 35 °C in a 5 L fermenter. After whole-genome sequencing of E. cloacae KRICT-1, we found that two quinoprotein glucose dehydrogenases (GDH1 and GDH2) from this strain could produce LBA from lactose by recombinantly expressing these genes in Escherichia coli. Our present results clearly demonstrated that E. cloacae KRICT-1 can be a new promising host for the industrial production of LBA. | - |
dc.identifier.bibliographicCitation | ACS Food Science and Technology, v.2, no.1, pp.66 - 74 | - |
dc.identifier.doi | 10.1021/acsfoodscitech.1c00322 | - |
dc.identifier.scopusid | 2-s2.0-85127051441 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/62224 | - |
dc.language | 영어 | - |
dc.publisher | American Chemical Society | - |
dc.title | Isolation and Characterization of a New Superior Lactobionic Acid-Producing Bacterium, Enterobacter cloacae KRICT-1, from Environmental Soil Samples | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Enterobacter cloacae | - |
dc.subject.keywordAuthor | lactobionic acid | - |
dc.subject.keywordAuthor | quinoprotein glucose dehydrogenase | - |
dc.subject.keywordAuthor | screening | - |
dc.subject.keywordAuthor | superior LBA-producing ability | - |
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Tel : 052-217-1404 / Email : scholarworks@unist.ac.kr
Copyright (c) 2023 by UNIST LIBRARY. All rights reserved.
ScholarWorks@UNIST was established as an OAK Project for the National Library of Korea.