dc.citation.endPage |
43 |
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dc.citation.number |
1 |
- |
dc.citation.startPage |
35 |
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dc.citation.title |
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY |
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dc.citation.volume |
50 |
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dc.contributor.author |
KIM, MH |
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dc.contributor.author |
LEE, HJ |
- |
dc.contributor.author |
Min, Kyung-Tai |
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dc.date.accessioned |
2023-12-22T12:42:41Z |
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dc.date.available |
2023-12-22T12:42:41Z |
- |
dc.date.created |
2014-09-15 |
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dc.date.issued |
1995-01 |
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dc.description.abstract |
Total synthesis of human Β-endorphin gene has been designed for the expression in bacterial system. Eight individual oligonucleotides corresponding to the Β-endorphin gene were chemically synthesized and joined through the enzyme-catalyzed reaction. The final yield of the 111-nucleotide-long synthetic Β-endorphin gene construct was about 10% of the total oligonucleotide used. The synthetic human Β-endorphin gene was cloned into the bacterium Escherichia coli, using pUC8 vector and shown to have the correct nucleotide sequences as designed. |
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dc.identifier.bibliographicCitation |
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.50, no.1, pp.35 - 43 |
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dc.identifier.doi |
10.1007/BF02788038 |
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dc.identifier.issn |
0273-2289 |
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dc.identifier.scopusid |
2-s2.0-0029201458 |
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dc.identifier.uri |
https://scholarworks.unist.ac.kr/handle/201301/6108 |
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dc.identifier.url |
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0029201458 |
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dc.identifier.wosid |
A1995QF57100003 |
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dc.language |
영어 |
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dc.publisher |
HUMANA PRESS INC |
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dc.title |
CHEMICAL SYNTHESIS AND CLONING OF HUMAN BETA-ENDORPHIN GENE IN ESCHERICHIA-COLI |
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dc.type |
Article |
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dc.description.journalRegisteredClass |
scopus |
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