Full metadata record
DC Field | Value | Language |
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dc.citation.startPage | 960907 | - |
dc.citation.title | FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY | - |
dc.citation.volume | 10 | - |
dc.contributor.author | Sathesh-Prabu, Chandran | - |
dc.contributor.author | Tiwari, Rameshwar | - |
dc.contributor.author | Lee, Sung Kuk | - |
dc.date.accessioned | 2023-12-21T13:45:43Z | - |
dc.date.available | 2023-12-21T13:45:43Z | - |
dc.date.created | 2022-09-14 | - |
dc.date.issued | 2022-08 | - |
dc.description.abstract | In this study, we developed a levulinic acid (LA)-inducible and antibiotic-free plasmid system mediated by HpdR/P- hpdH and infA-complementation to produce 4-hydroxyvaleric acid (4-HV) from LA in an engineered Escherichia coli strain. The system was efficiently induced by the addition of the LA substrate and resulted in tight dose-dependent control and fine-tuning of gene expression. By engineering the 5 & PRIME; untranslated region (UTR) of hpdR mRNA, the gene expression of green fluorescent protein (GFP) increased by at least two-fold under the hpdH promoter. Furthermore, by evaluating the robustness and plasmid stability of the proposed system, the engineered strain, IRV750f, expressing the engineered 3-hydroxybutyrate dehydrogenase (3HBDH*) and formate dehydrogenase (CbFDH), produced 82 g/L of 4-HV from LA, with a productivity of 3.4 g/L/h and molar conversion of 92% in the fed-batch cultivation (5 L fermenter) without the addition of antibiotics or external inducers. Overall, the reported system was highly beneficial for the large-scale and cost-effective microbial production of value-added products and bulk chemicals from the renewable substrate, LA. | - |
dc.identifier.bibliographicCitation | FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, v.10, pp.960907 | - |
dc.identifier.doi | 10.3389/fbioe.2022.960907 | - |
dc.identifier.issn | 2296-4185 | - |
dc.identifier.scopusid | 2-s2.0-85136560514 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/60063 | - |
dc.identifier.wosid | 000843836800001 | - |
dc.language | 영어 | - |
dc.publisher | FRONTIERS MEDIA SA | - |
dc.title | Substrate-inducible and antibiotic-free high-level 4-hydroxyvaleric acid production in engineered Escherichia coli | - |
dc.type | Article | - |
dc.description.isOpenAccess | TRUE | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology; Multidisciplinary Sciences | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology; Science & Technology - Other Topics | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | 4-hydroxyvaleric acid | - |
dc.subject.keywordAuthor | antibiotic-free | - |
dc.subject.keywordAuthor | promoter | - |
dc.subject.keywordAuthor | inducible expression | - |
dc.subject.keywordAuthor | levulinic acid | - |
dc.subject.keywordPlus | LEVULINIC ACID | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | GAMMA-VALEROLACTONE | - |
dc.subject.keywordPlus | PLATFORM | - |
dc.subject.keywordPlus | CHEMICALS | - |
dc.subject.keywordPlus | DESIGN | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | TOOLS | - |
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