Full metadata record
DC Field | Value | Language |
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dc.citation.startPage | 939248 | - |
dc.citation.title | FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY | - |
dc.citation.volume | 10 | - |
dc.contributor.author | Tiwari, Rameshwar | - |
dc.contributor.author | Sathesh-Prabu, Chandran | - |
dc.contributor.author | Lee, Sung Kuk | - |
dc.date.accessioned | 2023-12-21T13:45:40Z | - |
dc.date.available | 2023-12-21T13:45:40Z | - |
dc.date.created | 2022-09-14 | - |
dc.date.issued | 2022-08 | - |
dc.description.abstract | The present study elaborates on the propionic acid (PA) production by the well-known microbial cell factory Pseudomonas putida EM42 and its capacity to utilize biomass-derived levulinic acid (LA). Primarily, the P. putida EM42 strain was engineered to produce PA by deleting the methylcitrate synthase (PrpC) and propionyl-CoA synthase (PrpE) genes. Subsequently, a LA-inducible expression system was employed to express yciA (encoding thioesterase) from Haemophilus influenzae and ygfH (encoding propionyl-CoA: succinate CoA transferase) from Escherichia coli to improve the PA production by up to 10-fold under flask scale cultivation. The engineered P. putida EM42:& UDelta;CE:yciA:ygfH was used to optimize the bioprocess to further improve the PA production titer. Moreover, the fed-batch fermentation performed under optimized conditions in a 5 L bioreactor resulted in the titer, productivity, and molar yield for PA production of 26.8 g/L, 0.3 g/L/h, and 83%, respectively. This study, thus, successfully explored the LA catabolic pathway of P. putida as an alternative route for the sustainable and industrial production of PA from LA. | - |
dc.identifier.bibliographicCitation | FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, v.10, pp.939248 | - |
dc.identifier.doi | 10.3389/fbioe.2022.939248 | - |
dc.identifier.issn | 2296-4185 | - |
dc.identifier.scopusid | 2-s2.0-85136570472 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/60062 | - |
dc.identifier.wosid | 000844046200001 | - |
dc.language | 영어 | - |
dc.publisher | FRONTIERS MEDIA SA | - |
dc.title | Bioproduction of propionic acid using levulinic acid by engineered Pseudomonas putida | - |
dc.type | Article | - |
dc.description.isOpenAccess | TRUE | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology; Multidisciplinary Sciences | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology; Science & Technology - Other Topics | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | levulinic acid | - |
dc.subject.keywordAuthor | propionic acid | - |
dc.subject.keywordAuthor | Pseudomonas putida | - |
dc.subject.keywordAuthor | glycerol | - |
dc.subject.keywordAuthor | metabolic engineering | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | STRATEGIES | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | GLYCEROL | - |
dc.subject.keywordPlus | BIOMASS | - |
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