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Joo, Jinmyoung
Laboratory for Advanced Biomaterials and Translational Medicine
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dc.citation.startPage 911614 -
dc.citation.title FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY -
dc.citation.volume 10 -
dc.contributor.author Son, Boram -
dc.contributor.author Yoon, Hyungro -
dc.contributor.author Ryu, Jina -
dc.contributor.author Lee, Haein -
dc.contributor.author Joo, Jinmyoung -
dc.contributor.author Park, Hee Ho -
dc.contributor.author Park, Tai Hyun -
dc.date.accessioned 2023-12-21T13:50:46Z -
dc.date.available 2023-12-21T13:50:46Z -
dc.date.created 2022-08-24 -
dc.date.issued 2022-07 -
dc.description.abstract Induced pluripotent stem cells (iPSCs) have intrinsic properties, such as self-renewal ability and pluripotency, which are also shown in embryonic stem cells (ESCs). The challenge of improving the iPSC generation efficiency has been an important issue and there have been many attempts to develop iPSC generation methods. In this research, we added Lin28, known as one of the reprogramming factors, in the form of a soluble recombinant protein from E. coli to improve the efficiency of human iPSC (hiPSC) generation, in respect of alkaline phosphatase (AP)-positive colonies. To deliver Lin28 inside the cells, we generated a soluble Lin28-30Kc19 fusion protein, with 30Kc19 at the C-terminal domain of Lin28. 30Kc19, a silkworm hemolymph-derived protein, was fused due to its cell-penetrating and protein-stabilizing properties. The Lin28-30Kc19 was treated to human dermal fibroblasts (HDFs), in combination with four defined reprogramming factors (Oct4, Sox2, c-Myc, and Klf4). After 14 days of cell culture, we confirmed the generated hiPSCs through AP staining. According to the results, the addition of Lin28-30Kc19 increased the number and size of generated AP-positive hiPSC colonies. Through this research, we anticipate that this recombinant protein would be a valuable material for increasing the efficiency of hiPSC generation and for enhancing the possibility as a substitute of the conventional method. -
dc.identifier.bibliographicCitation FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, v.10, pp.911614 -
dc.identifier.doi 10.3389/fbioe.2022.911614 -
dc.identifier.issn 2296-4185 -
dc.identifier.scopusid 2-s2.0-85135447793 -
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/59176 -
dc.identifier.wosid 000837300200001 -
dc.language 영어 -
dc.publisher FRONTIERS MEDIA SA -
dc.title Enhanced efficiency of generating human-induced pluripotent stem cells using Lin28-30Kc19 fusion protein -
dc.type Article -
dc.description.isOpenAccess TRUE -
dc.relation.journalWebOfScienceCategory Biotechnology & Applied Microbiology; Multidisciplinary Sciences -
dc.relation.journalResearchArea Biotechnology & Applied Microbiology; Science & Technology - Other Topics -
dc.type.docType Article -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.subject.keywordAuthor human induced pluripotent stem cells (hiPSCs) -
dc.subject.keywordAuthor 30Kc19 -
dc.subject.keywordAuthor Lin28 -
dc.subject.keywordAuthor fusion protein -
dc.subject.keywordAuthor soluble -
dc.subject.keywordPlus MAJOR PLASMA-PROTEINS -
dc.subject.keywordPlus 30KC19 PROTEIN -
dc.subject.keywordPlus DELIVERY-SYSTEM -
dc.subject.keywordPlus EXPRESSION -
dc.subject.keywordPlus TRANSDUCTION -
dc.subject.keywordPlus PEPTIDE -
dc.subject.keywordPlus LIN28 -
dc.subject.keywordPlus SUPPLEMENTATION -
dc.subject.keywordPlus STABILIZATION -
dc.subject.keywordPlus MECHANISM -

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