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Cho, Yoon-Kyoung
Integrated Nano-Biotech Lab (INBL)
Research Interests
  • Microfluidics, Lab-on-a-chip, personalized biomedical diagnostics, nanobioengineering

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Characterization of DNA immobilization and subsequent hybridization using in situ quartz crystal microbalance, fluorescence spectroscopy, and surface plasmon resonance

Cited 45 times inthomson ciCited 41 times inthomson ci
Title
Characterization of DNA immobilization and subsequent hybridization using in situ quartz crystal microbalance, fluorescence spectroscopy, and surface plasmon resonance
Author
Cho, Yoon-KyoungKim, SKim, YALim, HKLee, KYoon, DSLim, GPak, YEHa, THKim, K
Issue Date
2004-10
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Citation
JOURNAL OF COLLOID AND INTERFACE SCIENCE, v.278, no.1, pp.44 - 52
Abstract
We have characterized the immobilization of thiol-modified oligomers on Au surfaces and subsequent hybridization with a perfectly matched or single-base mismatched target using a quartz crystal microbalance (QCM) and fluorescence spectroscopy. The surface density of immobilized probe molecules and the hybridization efficiency depending on the type of buffer and salt concentration were investigated. We observed some ambiguities in surface coverage deduced from QCM measurement and adopted a complementary fluorescence displacement method. Direct comparison of surface coverage deduced from frequency change in QCM measurement and determined by the fluorescence exchange reaction revealed that QCM results are highly overestimated and the amount of overestimation strongly depends on the type of buffer and the structure of the film. Discrimination capability of the surface attached 15-mer probe was also examined using a single-base mismatched target at various hybridization temperatures. Hybridization efficiency depending on the type of single base mismatch was investigated using surface plasmon resonance (SPR).
URI
https://scholarworks.unist.ac.kr/handle/201301/5897
URL
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=4143113539
DOI
10.1016/j.jcis.2004.05.008
ISSN
0021-9797
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BME_Journal Papers
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