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Kang, Sebyung
Protein Nanobio Lab.
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Highly sensitive pregnancy test kit via oriented antibody conjugation on brush-type ligand-coated quantum beads

Author(s)
Kim, SunghwanAhn, YujinBae, YoonjiWoo, SunyoungPark, JungsikHan, Im kyungKim, HyunhongEom, SoominKang, SebyungJung, WoonggyuPark, Jongnam
Issued Date
2022-10
DOI
10.1016/j.bios.2022.114441
URI
https://scholarworks.unist.ac.kr/handle/201301/58875
Fulltext
https://www.sciencedirect.com/science/article/pii/S095656632200481X
Citation
BIOSENSORS & BIOELECTRONICS, v.213, pp.114441
Abstract
Lateral flow assays (LFA) enable development of portable and rapid diagnostic kits; however, their capacity to detect low levels of disease markers remains poor. Here, we report a highly sensitive pregnancy test kit as a proof of concept, by combining brush-type ligand-coated quantum beads (B-type QBs) and nanobody, which can control the antibody orientation and enhance sensitivity. The brush-type ligand provided excellent dispersion stability and high-binding capacity toward antibody. Fc-binding nanobody increased the antigen-binding capacity of conjugated antibodies on the B-type QBs. To facilitate convenient acquisition of the LFA results, we developed a smartphone-based reader with a 3D-printed optical imaging module, and validated the diagnostic performance of the sensing platform. The pregnancy test kit achieved a 5.1 pg mL−1 limit of detection, corresponding to the levels for early-stage detection of heart disease and malaria. Our LFA application can potentially be expanded to diagnosis other diseases by simply changing the antibody pair in the kit.
Publisher
Pergamon Press Ltd.
ISSN
0956-5663
Keyword (Author)
Pregnancy test kitBrush-type ligandQuantum beadAntibody orientationPortable detectorAndroid application
Keyword
POLY(ACRYLIC ACID) BRUSHESLATERAL FLOW IMMUNOASSAYQUANTITATIVE DETECTIONGOLD NANOPARTICLESIMMUNOCHROMATOGRAPHIC ASSAYULTRASENSITIVE DETECTIONDOT NANOBEADSIMMOBILIZATIONPROTEINSNANOSPHERES

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