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Cho, Yoon-Kyoung
Integrated Nano-Biotech Lab (INBL)
Research Interests
  • Microfluidics, Lab-on-a-chip, personalized biomedical diagnostics, nanobioengineering

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Clinical evaluation of micro-scale chip-based PCR system for rapid detection of hepatitis B virus

Cited 51 times inthomson ciCited 59 times inthomson ci
Title
Clinical evaluation of micro-scale chip-based PCR system for rapid detection of hepatitis B virus
Author
Cho, Yoon-KyoungKim, JLee, YKim, YANamkoong, KLim, HOh, KWKim, SHan, JPark, CPak, YEKi, CSChoi, JRMyeong, HKKo, C
Issue Date
2006-05
Publisher
ELSEVIER ADVANCED TECHNOLOGY
Citation
BIOSENSORS & BIOELECTRONICS, v.21, no.11, pp.2161 - 2169
Abstract
The polymerase chain reaction (PCR) is widely used to amplify a small amount of DNA in samples for genetic analysis. Rapid and accurate amplification is prerequisite for broad applications including molecular diagnostics of diseases, food safety, and biological warfare tests. We have developed a rapid real-time micro-scale chip-based PCR system, which consists of six individual thermal cycling modules capable of independent control of PCR protocols. The PCR volume is 1 mu l and it takes less than 20 min to complete 40 thermal cycles. To test utility of a chip-based PCR system as a molecular diagnostic device, we have conducted the first large-scale clinical evaluation study. Three independent clinical evaluation studies (n=563) for screening the hepatitis B virus (HBV) infection, the Most Popular social epidemic disease in Asia, showed an excellent sensitivity, e.g. 94%, and specificity, e.g. 93%, demonstrating micro-scale chip-based PCR can be applied in molecular diagnostics.
URI
https://scholarworks.unist.ac.kr/handle/201301/5881
URL
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33646471069
DOI
10.1016/j.bios.2005.10.005
ISSN
0956-5663
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BME_Journal Papers
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