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DC Field | Value | Language |
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dc.citation.endPage | 212 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 205 | - |
dc.citation.title | COLLOIDS AND SURFACES B-BIOINTERFACES | - |
dc.citation.volume | 34 | - |
dc.contributor.author | Micic, M | - |
dc.contributor.author | Hu, DH | - |
dc.contributor.author | Suh, Yung Doug | - |
dc.contributor.author | Newton, G | - |
dc.contributor.author | Romine, M | - |
dc.contributor.author | Lu, HP | - |
dc.date.accessioned | 2023-12-22T11:06:32Z | - |
dc.date.available | 2023-12-22T11:06:32Z | - |
dc.date.created | 2022-01-24 | - |
dc.date.issued | 2004-04 | - |
dc.description.abstract | We report on imaging living bacterial cells by using a correlated tapping-mode atomic force microscopy (AFM) and confocal fluorescence lifetime imaging microscopy (FLIM). For optimal imaging of Gram-negative Shewanella oneidensis MR-1 cells, we explored different methods of bacterial sample preparation, Such as spreading the cells on poly-L-lysine coated surfaces or agarose gel coated Surfaces. We have found that the agarose gel containing 99% ammonium acetate buffer can provide sufficient local aqueous environment for single bacterial cells. Furthermore, the cell surface topography can be characterized by tapping-mode in-air AFM imaging for the single bacterial cells that are partially embedded. Using in-air rather than under-water AFM imaging of the living cells significantly enhanced the contrast and signal-to-noise ratio of the AFM images. Near-field AFM-tip-enhanced fluorescence lifetime imaging (AFM-FLIM) holds high promise on obtaining, fluorescence images beyond optical diffraction limited spatial resolution. We have previously demonstrated near-field AFM-FLIM imaging of polymer beads beyond diffraction limited spatial resolution. Here, as the first step of applying AFM-FLIM on imaging bacterial living cells, we demonstrated a correlated and consecutive AFM topographic imaging, fluorescence intensity imaging, and FLIM imaging of living bacterial cells to characterize cell polarity. (C) 2004 Elsevier B.V. All rights reserved. | - |
dc.identifier.bibliographicCitation | COLLOIDS AND SURFACES B-BIOINTERFACES, v.34, no.4, pp.205 - 212 | - |
dc.identifier.doi | 10.1016/j.colsurfb.2003.10.020 | - |
dc.identifier.issn | 0927-7765 | - |
dc.identifier.scopusid | 2-s2.0-1842611396 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/58789 | - |
dc.identifier.url | https://linkinghub.elsevier.com/retrieve/pii/S0927776503002789 | - |
dc.identifier.wosid | 000220934000001 | - |
dc.language | 영어 | - |
dc.publisher | ELSEVIER | - |
dc.title | Correlated atomic force microscopy and fluorescence lifetime imaging of live bacterial cells | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Biophysics; Chemistry, Physical; Materials Science, Biomaterials | - |
dc.relation.journalResearchArea | Biophysics; Chemistry; Materials Science | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | atomic force microscopy (AFM) | - |
dc.subject.keywordAuthor | fluorescence lifetime imaging microscopy (FLIM) | - |
dc.subject.keywordAuthor | confocal microscopy | - |
dc.subject.keywordAuthor | cell wall | - |
dc.subject.keywordAuthor | bacteria | - |
dc.subject.keywordAuthor | flagella | - |
dc.subject.keywordAuthor | Shewanella oneidensis | - |
dc.subject.keywordPlus | RAMAN-SPECTROSCOPY | - |
dc.subject.keywordPlus | SURFACE | - |
dc.subject.keywordPlus | LOCALIZATION | - |
dc.subject.keywordPlus | ADHESION | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | GROWTH | - |
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