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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 510 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 503 | - |
dc.citation.title | TOXICOLOGICAL RESEARCH | - |
dc.citation.volume | 38 | - |
dc.contributor.author | Lee, Ha Neul | - |
dc.contributor.author | Kim, Seyl | - |
dc.contributor.author | Park, Sooah | - |
dc.contributor.author | Jung, Woonggyu | - |
dc.contributor.author | Kang, Jin Seok | - |
dc.date.accessioned | 2023-12-21T13:39:04Z | - |
dc.date.available | 2023-12-21T13:39:04Z | - |
dc.date.created | 2022-05-27 | - |
dc.date.issued | 2022-10 | - |
dc.description.abstract | Histopathological examination is important for the diagnosis of various diseases. Conventional histopathology provides a two-dimensional view of the tissues, and requires the tissue to be extracted, fixed, and processed using histotechnology techniques. However, there is an increasing need for three-dimensional (3D) images of structures in biomedical research. The objective of this study was to develop reliable, objective tools for visualizing and quantifying metastatic tumors in mouse lung using micro-computed tomography (micro-CT), optical coherence tomography (OCT), and field emission-scanning electron microscopy (FE-SEM). Melanoma cells were intravenously injected into the tail vein of 8-week-old C57BL/6 mice. The mice were euthanized at 2 or 4 weeks after injection. Lungs were fixed and examined by micro-CT, OCT, FE-SEM, and histopathological observation. Micro-CT clearly distinguished between tumor and normal cells in surface and deep lesions, thereby allowing 3D quantification of the tumor volume. OCT showed a clear difference between the tumor and surrounding normal tissues. FE-SEM clearly showed round tumor cells, mainly located in the alveolar wall and growing inside the alveoli. Therefore, whole-tumor 3D imaging successfully visualized the metastatic tumor and quantified its volume. This promising approach will allow for fast and label-free 3D phenotyping of diverse tissue structures. | - |
dc.identifier.bibliographicCitation | TOXICOLOGICAL RESEARCH, v.38, no.4, pp.503 - 510 | - |
dc.identifier.doi | 10.1007/s43188-022-00134-4 | - |
dc.identifier.issn | 1976-8257 | - |
dc.identifier.scopusid | 2-s2.0-85128818868 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/58652 | - |
dc.identifier.url | https://link.springer.com/article/10.1007/s43188-022-00134-4 | - |
dc.identifier.wosid | 000794921200001 | - |
dc.language | 영어 | - |
dc.publisher | KOREAN SOC TOXICOLOGY | - |
dc.title | Quantification and visualization of metastatic lung tumors in mice | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.identifier.kciid | ART002886212 | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.type.docType | Article; Early Access | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.subject.keywordAuthor | Mice | - |
dc.subject.keywordAuthor | Micro-computed tomography | - |
dc.subject.keywordAuthor | Optical coherence tomography | - |
dc.subject.keywordAuthor | Field emission-scanning electron microscopy | - |
dc.subject.keywordAuthor | Histopathology | - |
dc.subject.keywordPlus | OPTICAL COHERENCE TOMOGRAPHY | - |
dc.subject.keywordPlus | BURDEN | - |
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