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DC Field | Value | Language |
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dc.citation.number | B | - |
dc.citation.startPage | 126090 | - |
dc.citation.title | BIORESOURCE TECHNOLOGY | - |
dc.citation.volume | 344 | - |
dc.contributor.author | Le, Thai | - |
dc.contributor.author | Park, Sunghoon | - |
dc.date.accessioned | 2023-12-21T14:42:45Z | - |
dc.date.available | 2023-12-21T14:42:45Z | - |
dc.date.created | 2022-01-10 | - |
dc.date.issued | 2022-01 | - |
dc.description.abstract | Production of 2-hydroxybutyric acid (2-HBA) was attempted in recombinant Escherichia coli W3110 Atdh AilvIH (over)expressing a homologous and mutated threonine dehydratase (ilvA*) and a heterologous 2-ketobutyric acid (2-KBA) reductase from Alcaligenes eutrophus H16 (Ae_ldh). To prevent the degradation of 2-KBA, the aceE, poxB and pflB genes were deleted, and for blocking the 2-HBA degradation, the lldD and dld genes were disrupted. In addition, for efficient NADH regeneration/supply, a heterologous formate dehydrogenase from Candida boidinii (Cb_fdh) was overexpressed. Under anaerobic condition, E. coli W3110 Atdh AilvIH AaceE ApoxB AlldD Adld ApflB could produce > 400 mM 2-HBA in 33 h with the yield of - 0.95 mol/mol. Furthermore, by enhancing the expression of a mutant Cb_fdh, the titer could be increased to - 650 mM in 33 h. This study provides an efficient microbial cell factory for the bioconversion of threonine to 2-HBA with a high yield. | - |
dc.identifier.bibliographicCitation | BIORESOURCE TECHNOLOGY, v.344, no.B, pp.126090 | - |
dc.identifier.doi | 10.1016/j.biortech.2021.126090 | - |
dc.identifier.issn | 0960-8524 | - |
dc.identifier.scopusid | 2-s2.0-85120904061 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/58501 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0960852421014322?via%3Dihub | - |
dc.identifier.wosid | 000733229300006 | - |
dc.language | 영어 | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Development of efficient microbial cell factory for whole-cell bioconversion of L-threonine to 2-hydroxybutyric acid | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels | - |
dc.relation.journalResearchArea | Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | 2-ketobutyric acid | - |
dc.subject.keywordAuthor | 2-hydroxybutyric acid | - |
dc.subject.keywordAuthor | 2-ketobutyric acid degradation | - |
dc.subject.keywordAuthor | 2-hydroxybutyric acid degradation | - |
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