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Kim, Taesung
Microfluidics & Nanomechatronics Lab (μFNM)
Research Interests
  • Microfluidics & Nanofluidics
  • Nanoscale Transport Phenomena
  • MEMS & BioMEMS
  • Nanofabrication & Nanomechatronics


Patterned delivery and expression of gene constructs into zebrafish embryos using microfabricated interfaces

DC Field Value Language Bansal, Tushar ko Lenhart, Justin ko Kim, Taesung ko Duan, Cunming ko Maharbiz, Michel M ko 2014-09-11T06:55:14Z - 2014-09-11 ko 2009-06 ko
dc.identifier.citation BIOMEDICAL MICRODEVICES, v.11, no.3, pp.633 - 641 ko
dc.identifier.issn 1387-2176 ko
dc.identifier.uri -
dc.description.abstract We demonstrate a method which uses simple microfabrication and microfluidics to produce custom, shaped electroporators for the patterned delivery of foreign molecules into developing embryos. We show how these electroporators can be used to 'draw' two-dimensional patterns of tracer molecules, DNA and mRNA into the yolk and cells of zebrafish embryos (Danio rerio) at different stages of development. We demonstrate the successful delivery of patterns of Trypan Blue (normal dye), Texas Red (fluorescent dye), GFP-expressing DNA plasmids and GFP expressing mRNA constructs into both chorionated and dechorionated embryos. Both DNA and mRNA were expressed in the desired patterns subsequent to delivery. Square pulses of 10-20 V (0.20-0.40 kV/cm), 50-100 ms width were sufficient to create transient pores and introduce compounds from the late blastula period (3 hpf) to early pharyngula period (24 hpf) embryos. Using 24 hpf dechorionated embryos, we achieved a high survival of 91.3% and 89%, and a delivery efficiency of 38% and 50% for GFP-DNA and GFP-mRNA respectively. Lastly, we demonstrate the simultaneous delivery of different compounds into the developing embryo. ko
dc.description.statementofresponsibility close -
dc.language 영어 ko
dc.publisher SPRINGER ko
dc.title Patterned delivery and expression of gene constructs into zebrafish embryos using microfabricated interfaces ko
dc.type ARTICLE ko
dc.identifier.scopusid 2-s2.0-67349253118 ko
dc.identifier.wosid 000265440300013 ko
dc.type.rims ART ko
dc.description.wostc 8 *
dc.description.scopustc 9 * 2015-05-06 * 2014-09-11 *
dc.identifier.doi 10.1007/s10544-008-9273-5 ko
dc.identifier.url ko
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