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DC Field | Value | Language |
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dc.citation.startPage | 127106 | - |
dc.citation.title | BIORESOURCE TECHNOLOGY | - |
dc.citation.volume | 352 | - |
dc.contributor.author | Lee, Siseon | - |
dc.contributor.author | Jung, Ye Jean | - |
dc.contributor.author | Park, Si Jae | - |
dc.contributor.author | Ryu, Mi-Hee | - |
dc.contributor.author | Kim, Joo Eon | - |
dc.contributor.author | Song, Hye Min | - |
dc.contributor.author | Kang, Kyoung Hee | - |
dc.contributor.author | Song, Bong Keun | - |
dc.contributor.author | Sung, Bong Hyun | - |
dc.contributor.author | Kim, Yong Hwan | - |
dc.contributor.author | Kim, Hee Taek | - |
dc.contributor.author | Joo, Jeong Chan | - |
dc.date.accessioned | 2023-12-21T14:17:53Z | - |
dc.date.available | 2023-12-21T14:17:53Z | - |
dc.date.created | 2022-04-04 | - |
dc.date.issued | 2022-04 | - |
dc.description.abstract | Lignin valorization depends on microbial upcycling of various aromatic compounds in the form of a complex mixture, including p-coumaric acid and ferulic acid. In this study, an engineered Pseudomonas putida strain utilizing lignin-derived monomeric compounds via biological funneling was developed to produce 2-pyrone-4,6-dicarboxylic acid (PDC), which has been considered a promising building block for bioplastics. The biosynthetic pathway for PDC production was established by introducing the heterologous ligABC gene under the promoter Ptac in a strain lacking pcaGH genes to accumulate a precursor of PDC, i.e., protocatechuic acid. Based on the culture optimization, fed-batch fermentation of the final strain resulted in 22.7 g/L PDC with a molar yield of 1.0 mol/mol and productivity of 0.21 g/L/h. Subsequent purification of PDC at high purity was successfully implemented, which was consequently applied for the novel polyester. | - |
dc.identifier.bibliographicCitation | BIORESOURCE TECHNOLOGY, v.352, pp.127106 | - |
dc.identifier.doi | 10.1016/j.biortech.2022.127106 | - |
dc.identifier.issn | 0960-8524 | - |
dc.identifier.scopusid | 2-s2.0-85127811784 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/57733 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0960852422004357?via%3Dihub | - |
dc.identifier.wosid | 000793757300011 | - |
dc.language | 영어 | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Microbial production of 2-pyrone-4,6-dicarboxylic acid from lignin derivatives in an engineered Pseudomonas putida and its application for the synthesis of bio-based polyester | - |
dc.type | Article | - |
dc.description.isOpenAccess | TRUE | - |
dc.relation.journalWebOfScienceCategory | Agricultural Engineering;Biotechnology & Applied Microbiology;Energy & Fuels | - |
dc.relation.journalResearchArea | AgricultureㅣBiotechnology & Applied Microbiology;Energy & Fuels | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | 2-pyrone-4 | - |
dc.subject.keywordAuthor | 6-dicarboxylic acid | - |
dc.subject.keywordAuthor | Bio-based polyester | - |
dc.subject.keywordAuthor | Biological funneling | - |
dc.subject.keywordAuthor | Lignin | - |
dc.subject.keywordAuthor | Pseudomonas putida | - |
dc.subject.keywordPlus | FORMALDEHYDE | - |
dc.subject.keywordPlus | MONOMER | - |
dc.subject.keywordPlus | BIOMASS | - |
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