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Kim, Yong Hwan
Enzyme and Protein Engineering Lab.
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dc.citation.startPage 127106 -
dc.citation.title BIORESOURCE TECHNOLOGY -
dc.citation.volume 352 - Lee, Siseon - Jung, Ye Jean - Park, Si Jae - Ryu, Mi-Hee - Kim, Joo Eon - Song, Hye Min - Kang, Kyoung Hee - Song, Bong Keun - Sung, Bong Hyun - Kim, Yong Hwan - Kim, Hee Taek - Joo, Jeong Chan - 2023-12-21T14:17:53Z - 2023-12-21T14:17:53Z - 2022-04-04 - 2022-04 -
dc.description.abstract Lignin valorization depends on microbial upcycling of various aromatic compounds in the form of a complex mixture, including p-coumaric acid and ferulic acid. In this study, an engineered Pseudomonas putida strain utilizing lignin-derived monomeric compounds via biological funneling was developed to produce 2-pyrone-4,6-dicarboxylic acid (PDC), which has been considered a promising building block for bioplastics. The biosynthetic pathway for PDC production was established by introducing the heterologous ligABC gene under the promoter Ptac in a strain lacking pcaGH genes to accumulate a precursor of PDC, i.e., protocatechuic acid. Based on the culture optimization, fed-batch fermentation of the final strain resulted in 22.7 g/L PDC with a molar yield of 1.0 mol/mol and productivity of 0.21 g/L/h. Subsequent purification of PDC at high purity was successfully implemented, which was consequently applied for the novel polyester. -
dc.identifier.bibliographicCitation BIORESOURCE TECHNOLOGY, v.352, pp.127106 -
dc.identifier.doi 10.1016/j.biortech.2022.127106 -
dc.identifier.issn 0960-8524 -
dc.identifier.scopusid 2-s2.0-85127811784 -
dc.identifier.uri -
dc.identifier.url -
dc.identifier.wosid 000793757300011 -
dc.language 영어 -
dc.publisher ELSEVIER SCI LTD -
dc.title Microbial production of 2-pyrone-4,6-dicarboxylic acid from lignin derivatives in an engineered Pseudomonas putida and its application for the synthesis of bio-based polyester -
dc.type Article -
dc.description.isOpenAccess TRUE -
dc.relation.journalWebOfScienceCategory Agricultural Engineering;Biotechnology & Applied Microbiology;Energy & Fuels -
dc.relation.journalResearchArea AgricultureㅣBiotechnology & Applied Microbiology;Energy & Fuels -
dc.type.docType Article -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.subject.keywordAuthor 2-pyrone-4 -
dc.subject.keywordAuthor 6-dicarboxylic acid -
dc.subject.keywordAuthor Bio-based polyester -
dc.subject.keywordAuthor Biological funneling -
dc.subject.keywordAuthor Lignin -
dc.subject.keywordAuthor Pseudomonas putida -
dc.subject.keywordPlus FORMALDEHYDE -
dc.subject.keywordPlus MONOMER -
dc.subject.keywordPlus BIOMASS -


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