Precision targeting tumor cells using cancer-specific InDel mutations with CRISPR-Cas9
Cited 0 times inCited 0 times in
- Precision targeting tumor cells using cancer-specific InDel mutations with CRISPR-Cas9
- Kwon, Taejoon; Ra, Jae Sun; Lee, Soyoung; Baek, In-Joon; Khim, Keon Woo; Lee, Eun A; Song, Eun Kyung; Otarbayev, Daniyar; Jung, Woojae; Park, Yong Hwan; Wie, Minwoo; Bae, Juyoung; Cheng, Himchan; Park, Jun Hong; Kim, Namwoo; Seo, Yuri; Yun, Seongmin; Kim, Ha Eun; Moon, Hyo Eun; Paek, Sun Ha; Park, Tae Joo; Park, Young Un; Rhee, Hwanseok; Choi, Jang Hyun; Cho, Seung Woo; Myung, Kyungjae
- Issue Date
- NATL ACAD SCIENCES
- PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.119, no.9, pp.e2103532119
- An ideal cancer therapeutic strategy involves the selective killing of cancer cells without affecting the surrounding normal cells. However, researchers have failed to develop such methods for achieving selective cancer cell death because of shared features between cancerous and normal cells. In this study, we have developed a therapeutic strategy called the cancer-specific insertions–deletions (InDels) attacker (CINDELA) to selectively induce cancer cell death using the CRISPR-Cas system. CINDELA utilizes a previously unexplored idea of introducing CRISPR-mediated DNA double-strand breaks (DSBs) in a cancer-specific fashion to facilitate specific cell death. In particular, CINDELA targets multiple InDels with CRISPR-Cas9 to produce many DNA DSBs that result in cancer-specific cell death. As a proof of concept, we demonstrate here that CINDELA selectively kills human cancer cell lines, xenograft human tumors in mice, patient-derived glioblastoma, and lung patient-driven xenograft tumors without affecting healthy human cells or altering mouse growth.
- Appears in Collections:
- BIO_Journal Papers
- Files in This Item:
- There are no files associated with this item.
can give you direct access to the published full text of this article. (UNISTARs only)
Show full item record
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.