dc.citation.endPage |
A1339 |
- |
dc.citation.number |
9 |
- |
dc.citation.startPage |
A1339 |
- |
dc.citation.title |
FASEB JOURNAL |
- |
dc.citation.volume |
11 |
- |
dc.contributor.author |
Suh, Pann-Ghill |
- |
dc.contributor.author |
Kim, MJ |
- |
dc.contributor.author |
Min, DS |
- |
dc.contributor.author |
Ryu, SH |
- |
dc.date.accessioned |
2023-12-22T12:37:14Z |
- |
dc.date.available |
2023-12-22T12:37:14Z |
- |
dc.date.created |
2014-09-02 |
- |
dc.date.issued |
1997-07 |
- |
dc.description.abstract |
Previously reported the cloning of a cDNA encoding rat phospholipase C-b4 (PLC-b4), a mammalian homolog of Drosophila NorpA PLC (Kim, M.J., Bahk, Y.Y., Min, 1).S., Lee, S.-J., Ryu, S.H., and Sub, P.-G. (1993) Biochem. Biophys. Res. Commun. 194,706-712). We now report the isolation of a splice variant (PLC-b4b). PLC-b4b is identical to the 130 kDa PLC-b4 (PLC-b4a) except that the carboxyl-terminal 162 amino acids of PLC-b4a are replaced with 10 distinct amino acids. The existence of PLC-b4b transcripts in the rat brain was dernonstrated by RT-PCR analysis. Immunological -analysis using polyclonal antibody specific for PLC-b4b revealed that this splice variant exists in the rat brain cytosol. To investigate functional differences between the two forms of PLC-b4, transient expression studies in COS-7 cells were conducted. We found that PLC-b4a is mainly localized in the particulate fraction of the cell and can be activated by Gaq, whereas PLC-b4b is exclusively localized in the soluble fraction and can not be activated by Gaq. In addition both PLC-b4a and PLC-b4b were not activated by G-protein bg-subunits purified from rat brain. These results suggest that the carboxyl-terminal 162 amino acids of PLC-b4a might be required for association of the protein with the particulate fraction and activation by Gaq and suggest that PLC-b4b may be regulated by a different mechanism and play a distinct role in PLC-mediated signal transduction. |
- |
dc.identifier.bibliographicCitation |
FASEB JOURNAL, v.11, no.9, pp.A1339 - A1339 |
- |
dc.identifier.issn |
0892-6638 |
- |
dc.identifier.scopusid |
2-s2.0-33750243391 |
- |
dc.identifier.uri |
https://scholarworks.unist.ac.kr/handle/201301/5705 |
- |
dc.identifier.url |
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33750243391 |
- |
dc.identifier.wosid |
000073305603301 |
- |
dc.language |
영어 |
- |
dc.publisher |
FEDERATION AMER SOC EXP BIOL |
- |
dc.title |
Cloning of a splice variant of rat phospholipase C-beta4 which is not activated by Gaq |
- |
dc.type |
Article |
- |
dc.description.journalRegisteredClass |
scopus |
- |