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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 52503 | - |
dc.citation.number | 52 | - |
dc.citation.startPage | 52497 | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 278 | - |
dc.contributor.author | Rong, R | - |
dc.contributor.author | Ahn, JY | - |
dc.contributor.author | Chen, P | - |
dc.contributor.author | Suh, Pann-Ghill | - |
dc.contributor.author | Ye, KQ | - |
dc.date.accessioned | 2023-12-22T11:08:17Z | - |
dc.date.available | 2023-12-22T11:08:17Z | - |
dc.date.created | 2014-09-03 | - |
dc.date.issued | 2003-12 | - |
dc.description.abstract | Phospholipase C-gamma1( PLC-gamma1) hydrolyzes phosphatidylinositol 4,5-bisphosphate to the second messengers inositol 1,4,5-trisphosphate and diacylglycerol (DAG). PLC-gamma1 is implicated in a variety of cellular signalings and processes including mitogenesis and calcium entry. However, numerous studies demonstrate that the lipase activity is not required for PLC-gamma1 to mediate these events. Here, we report that the phospholipase activity of PLC-gamma1 plays an essential role in nerve growth factor (NGF)-triggered Raf/MEK/MAPK pathway activation in PC12 cells. Employing PC12 cells stably transfected with an inducible form of wild-type PLC-gamma1 or lipase inactive PLC-gamma1 with histidine 335 mutated into glutamine in the catalytic domain, we show that NGF provokes robust activation of MAP kinase in wild-type but not in lipase inactive cells. Both Ras/C-Raf/MEK1 and Rap1/B-Raf/MEK1 pathways are intact in the wild-type cells. By contrast, these signaling cascades are diminished in the mutant cells. Pretreatment with cell permeable DAG analog 1-oleyl-2-acetylglycerol rescues the MAP kinase pathway activation in the mutant cells. These observations indicate that the lipase activity of PLC-gamma1 mediates NGF-regulated MAPK signaling upstream of Ras/Rap1 activation probably through second messenger DAG-activated Ras and Rap-GEFs. | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.278, no.52, pp.52497 - 52503 | - |
dc.identifier.doi | 10.1074/jbc.M306744200 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.scopusid | 2-s2.0-0347993075 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/5680 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0347993075 | - |
dc.identifier.wosid | 000187480700064 | - |
dc.language | 영어 | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.title | Phospholipase activity of phospholipase C-gamma 1 is required for nerve growth factor-regulated MAP kinase signaling cascade in PC12 cells | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
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