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DC Field | Value | Language |
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dc.citation.title | FRONTIERS IN NEUROANATOMY | - |
dc.citation.volume | 15 | - |
dc.contributor.author | Kim, Gyeong Tae | - |
dc.contributor.author | Bahn, Sangkyu | - |
dc.contributor.author | Kim, Nari | - |
dc.contributor.author | Choi, Joon Ho | - |
dc.contributor.author | Kim, Jinseop S. | - |
dc.contributor.author | Rah, Jong-Cheol | - |
dc.date.accessioned | 2023-12-21T15:07:06Z | - |
dc.date.available | 2023-12-21T15:07:06Z | - |
dc.date.created | 2022-01-04 | - |
dc.date.issued | 2021-11 | - |
dc.description.abstract | Critical determinants of synaptic functions include subcellular locations, input sources, and specific molecular characteristics. However, there is not yet a reliable and efficient method that can detect synapses. Electron microscopy is a gold-standard method to detect synapses due to its exceedingly high spatial resolution. However, it requires laborious and time-consuming sample preparation and lengthy imaging time with limited labeling methods. Recent advances in various fluorescence microscopy methods have highlighted fluorescence microscopy as a substitute for electron microscopy in reliable synapse detection in a large volume of neural circuits. In particular, array tomography has been verified as a useful tool for neural circuit reconstruction. To further improve array tomography, we developed a novel imaging method, called "structured illumination microscopy on the putative region of interest on ultrathin sections", which enables efficient and accurate detection of synapses-of-interest. Briefly, based on low-magnification conventional fluorescence microscopy images, synapse candidacy was determined. Subsequently, the coordinates of the regions with candidate synapses were imaged using super-resolution structured illumination microscopy. Using this system, synapses from the high-order thalamic nucleus, the posterior medial nucleus in the barrel cortex were rapidly and accurately imaged. | - |
dc.identifier.bibliographicCitation | FRONTIERS IN NEUROANATOMY, v.15 | - |
dc.identifier.doi | 10.3389/fnana.2021.759816 | - |
dc.identifier.issn | 1662-5129 | - |
dc.identifier.scopusid | 2-s2.0-85120523447 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/55908 | - |
dc.identifier.wosid | 000726150000001 | - |
dc.language | 영어 | - |
dc.publisher | FRONTIERS MEDIA SA | - |
dc.title | Efficient and Accurate Synapse Detection With Selective Structured Illumination Microscopy on the Putative Regions of Interest of Ultrathin Serial Sections | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Anatomy & Morphology; Neurosciences | - |
dc.relation.journalResearchArea | Anatomy & Morphology; Neurosciences & Neurology | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | structured illumination microscopy | - |
dc.subject.keywordAuthor | array tomography | - |
dc.subject.keywordAuthor | synapse location | - |
dc.subject.keywordAuthor | posterior medial nucleus | - |
dc.subject.keywordAuthor | barrel cortex | - |
dc.subject.keywordPlus | CEREBRAL-CORTEX | - |
dc.subject.keywordPlus | ORGANIZATION | - |
dc.subject.keywordPlus | RESOLUTION | - |
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