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DC Field | Value | Language |
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dc.citation.endPage | 116 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 110 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 32 | - |
dc.contributor.author | Kim, Doyun | - |
dc.contributor.author | Lee, Sung Kuk | - |
dc.date.accessioned | 2023-12-21T14:43:11Z | - |
dc.date.available | 2023-12-21T14:43:11Z | - |
dc.date.created | 2022-01-03 | - |
dc.date.issued | 2022-01 | - |
dc.description.abstract | Polyhydroxyalkanoates (PHAs) are emerging as alternatives to plastics by replacing fossil fuels with renewable raw substrates. Herein, we present the construction of engineered Escherichia coli strains to produce short-chain-length PHAs (scl-PHAs), including the monomers 4-hydroxyvalerate (4HV) and 3-hydroxyvalerate (3HV) produced from levulinic acid (LA). First, an E. coli strain expressing genes (lvaEDABC) from the levulinic acid metabolic pathway of Pseudomonas putida KT2440 was constructed to generate 4HV-CoA and 3HV-CoA. Second, both PhaAB enzymes from Cupriavidus necator H16 were expressed to supply 3-hydroxybutyrate (3HB)-CoA from acetyl-CoA. Finally, PHA synthase (PhaCCv) from Chromobacterium violaceum was introduced for the subsequent polymerization of these three monomers. The resulting E. coli strains produced four PHAs (w/w% of dry cell weight): 9.1 wt% P(4HV), 1.7 wt% P(3HV-co-4HV), 24.2 wt% P(3HB-co-4HV), and 35.6 wt% P(3HB-co-3HV-co-4HV). | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.32, no.1, pp.110 - 116 | - |
dc.identifier.doi | 10.4014/jmb.2108.08016 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.scopusid | 2-s2.0-85123870795 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/55885 | - |
dc.identifier.wosid | 000894021200002 | - |
dc.language | 영어 | - |
dc.publisher | 한국미생물·생명공학회 | - |
dc.title | Metabolic engineering of Escherichia coli for production of polyhydroxyalkanotes with valeric acids derived from levulinic acid | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology;Microbiology | - |
dc.identifier.kciid | ART002807821 | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology;Microbiology | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.subject.keywordAuthor | Levulinic acid | - |
dc.subject.keywordAuthor | Escherichia coli | - |
dc.subject.keywordAuthor | short-chain-length polyhydroxyalkanoates (scl-PHAs) | - |
dc.subject.keywordPlus | COMPLETE GENOME SEQUENCE | - |
dc.subject.keywordPlus | BIOTECHNOLOGICAL PRODUCTION | - |
dc.subject.keywordPlus | PSEUDOMONAS-PUTIDA | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | GENES | - |
dc.subject.keywordPlus | POLY(3-HYDROXYBUTYRATE) | - |
dc.subject.keywordPlus | POLYESTERS | - |
dc.subject.keywordPlus | PLATFORM | - |
dc.subject.keywordPlus | PATHWAY | - |
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