Full metadata record
DC Field | Value | Language |
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dc.citation.endPage | 662 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 653 | - |
dc.citation.title | WORLD JOURNAL OF MENS HEALTH | - |
dc.citation.volume | 40 | - |
dc.contributor.author | Kim, Seul Gi | - |
dc.contributor.author | You, Dalsan | - |
dc.contributor.author | Kim, Kyung | - |
dc.contributor.author | Aum, Joomin | - |
dc.contributor.author | Kim, Yu Seon | - |
dc.contributor.author | Jang, Myoung Jin | - |
dc.contributor.author | Moon, Kyung Hyun | - |
dc.contributor.author | Kang, Hyun-Wook | - |
dc.date.accessioned | 2023-12-21T13:39:16Z | - |
dc.date.available | 2023-12-21T13:39:16Z | - |
dc.date.created | 2021-10-04 | - |
dc.date.issued | 2022-10 | - |
dc.description.abstract | Purpose: Owing to the safety and cost effectiveness of conditioned medium (CM), its therapeutic effects have attracted signifi-cant attention from many researchers. To date, numerous studies have been conducted on CM; however, little has been done with regard to erectile dysfunction (ED). In this research, the potential of human mesenchymal stem cell-derived CM (MSC-CM) for the treatment of ED was investigated. Materials and Methods: A high concentration of MSC-CM was prepared through 3D spheroid culturing with bone marrow-derived MSCs and cut-off filtering. The composition of CM was analyzed using biochemical assays, and the effect of the preparation process on the quality of CM was investigated. The therapeutic effects of MSC-CM were evaluated through ani-mal studies using a cavernous nerve (CN) injury rat model. Results: 3D spheroid culturing afforded a 278-fold increase in the total protein content of CM, as compared to that from 2D cultures; the protein concentration increased by 19 times on increasing the centrifugation time for cut-off filtering. Biochemi-cal assays indicated that the CM contains various types of angiogenic, neurotrophic, and anti-inflammatory factors. Histologi -cal assay results showed that MSC-CM has angio-and neuro-trophic effect in a CN injury rat model in vivo, and these thera-peutic effects appear in a dose-dependent manner. Conclusions: The experimental results confirmed the therapeutic effect of MSC-CM in healing damaged cavernosal tissue and restoring erectile function. These results successfully demonstrated that MSC-CM has significant potential for the treatment of ED. | - |
dc.identifier.bibliographicCitation | WORLD JOURNAL OF MENS HEALTH, v.40, no.4, pp.653 - 662 | - |
dc.identifier.doi | 10.5534/wjmh.210121 | - |
dc.identifier.issn | 2287-4208 | - |
dc.identifier.scopusid | 2-s2.0-85123791529 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/54061 | - |
dc.identifier.url | https://wjmh.org/search.php?where=aview&id=10.5534/wjmh.210121&code=2074WJMH&vmode=FULL | - |
dc.identifier.wosid | 000742887400001 | - |
dc.language | 영어 | - |
dc.publisher | PUSAN NATL UNIV MEDICAL SCH | - |
dc.title | Therapeutic effect of human mesenchymal stem cell-conditioned medium on erectile dysfunction | - |
dc.type | Article | - |
dc.description.isOpenAccess | TRUE | - |
dc.relation.journalWebOfScienceCategory | Andrology; Health Care Sciences & Services; Urology & Nephrology | - |
dc.relation.journalResearchArea | Endocrinology & Metabolism; Health Care Sciences & Services; Urology & Nephrology | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.subject.keywordAuthor | Angiotrophic effect | - |
dc.subject.keywordAuthor | Conditioned medium | - |
dc.subject.keywordAuthor | Erectile dysfunction | - |
dc.subject.keywordAuthor | Mesenchymal stem cell | - |
dc.subject.keywordAuthor | Neurotrophic effect | - |
dc.subject.keywordPlus | CAVERNOUS NERVE INJURY | - |
dc.subject.keywordPlus | RAT MODEL | - |
dc.subject.keywordPlus | ENDOTHELIAL DYSFUNCTION | - |
dc.subject.keywordPlus | RECOVERY | - |
dc.subject.keywordPlus | TISSUE | - |
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