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Kang, Hyun-Wook
3D Biofabrication Lab.
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Therapeutic effect of human mesenchymal stem cell-conditioned medium on erectile dysfunction

Author(s)
Kim, Seul GiYou, DalsanKim, KyungAum, JoominKim, Yu SeonJang, Myoung JinMoon, Kyung HyunKang, Hyun-Wook
Issued Date
2022-10
DOI
10.5534/wjmh.210121
URI
https://scholarworks.unist.ac.kr/handle/201301/54061
Fulltext
https://wjmh.org/search.php?where=aview&id=10.5534/wjmh.210121&code=2074WJMH&vmode=FULL
Citation
WORLD JOURNAL OF MENS HEALTH, v.40, no.4, pp.653 - 662
Abstract
Purpose: Owing to the safety and cost effectiveness of conditioned medium (CM), its therapeutic effects have attracted signifi-cant attention from many researchers. To date, numerous studies have been conducted on CM; however, little has been done with regard to erectile dysfunction (ED). In this research, the potential of human mesenchymal stem cell-derived CM (MSC-CM) for the treatment of ED was investigated. Materials and Methods: A high concentration of MSC-CM was prepared through 3D spheroid culturing with bone marrow-derived MSCs and cut-off filtering. The composition of CM was analyzed using biochemical assays, and the effect of the preparation process on the quality of CM was investigated. The therapeutic effects of MSC-CM were evaluated through ani-mal studies using a cavernous nerve (CN) injury rat model. Results: 3D spheroid culturing afforded a 278-fold increase in the total protein content of CM, as compared to that from 2D cultures; the protein concentration increased by 19 times on increasing the centrifugation time for cut-off filtering. Biochemi-cal assays indicated that the CM contains various types of angiogenic, neurotrophic, and anti-inflammatory factors. Histologi -cal assay results showed that MSC-CM has angio-and neuro-trophic effect in a CN injury rat model in vivo, and these thera-peutic effects appear in a dose-dependent manner. Conclusions: The experimental results confirmed the therapeutic effect of MSC-CM in healing damaged cavernosal tissue and restoring erectile function. These results successfully demonstrated that MSC-CM has significant potential for the treatment of ED.
Publisher
PUSAN NATL UNIV MEDICAL SCH
ISSN
2287-4208
Keyword (Author)
Angiotrophic effectConditioned mediumErectile dysfunctionMesenchymal stem cellNeurotrophic effect
Keyword
CAVERNOUS NERVE INJURYRAT MODELENDOTHELIAL DYSFUNCTIONRECOVERYTISSUE

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