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Cho, Yoon-Kyoung
Integrated Nano-Biotech Lab (INBL)
Research Interests
  • Microfluidics, Lab-on-a-chip, personalized biomedical diagnostics, nanobioengineering

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Programmed exosome fusion for energy generation in living cells

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Title
Programmed exosome fusion for energy generation in living cells
Author
Kumar, SumitKarmacharya, MamataMichael, Issac J.Choi, YongjunKim, JunyoungKim, InUnCho, Yoon-Kyoung
Issue Date
2021-09
Publisher
NATURE PORTFOLIO
Citation
NATURE CATALYSIS, v.4, pp.763 - 774
Abstract
Biological membrane-enclosed organelles are fascinating examples of spatially confined nanoreactors for biocatalytic transformations such as cascade reactions involving multiple enzymes; however, the fabrication of their synthetic mimics remains a considerable challenge. Here we demonstrate supramolecular chemistry-based bridging of two membranes leading to controlled fusion of exosomes that act as nanoreactors for effective biocatalytic cascades, with prolonged functionality inside of living cells. Exosome membrane proteins were chemically engineered with a catechol moiety to drive fusion by supramolecular complexation to bridge the membranes. This strategy successfully encapsulated multiple enzymes and assembled the minimal electron transport chain in the plasma membrane, leading to tuneable, enhanced catalytic cascade activity capable of ATP synthesis inside of tissue spheroids. This nanoreactor was functional for many hours after uptake into living cells, showed successful penetration into tissue spheroids and repaired the damaged region by supplying ATP, all of which represent an advance in the mimicking of nature's own organelles.
URI
https://scholarworks.unist.ac.kr/handle/201301/53993
URL
https://www.nature.com/articles/s41929-021-00669-z
DOI
10.1038/s41929-021-00669-z
ISSN
2520-1158
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BME_Journal Papers
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