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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 166 | - |
dc.citation.startPage | 157 | - |
dc.citation.title | METABOLIC ENGINEERING | - |
dc.citation.volume | 66 | - |
dc.contributor.author | Wang, Qintao | - |
dc.contributor.author | Feng, Yanbin | - |
dc.contributor.author | Lu, Yandu | - |
dc.contributor.author | Xin, Yi | - |
dc.contributor.author | Shen, Chen | - |
dc.contributor.author | Wei, Li | - |
dc.contributor.author | Liu, Yuxue | - |
dc.contributor.author | Lv, Nana | - |
dc.contributor.author | Du, Xuefeng | - |
dc.contributor.author | Zhu, Wenqiang | - |
dc.contributor.author | Jeong, Byeong-ryool | - |
dc.contributor.author | Xue, Song | - |
dc.contributor.author | Xu, Jian | - |
dc.date.accessioned | 2023-12-21T15:39:58Z | - |
dc.date.available | 2023-12-21T15:39:58Z | - |
dc.date.created | 2021-06-26 | - |
dc.date.issued | 2021-07 | - |
dc.description.abstract | The chain length (CL) of fatty acids (FAs) is pivotal to oil property, yet to what extent it can be customized in industrial oleaginous microalgae is unknown. In Nannochloropsis oceanica, to modulate long-chain FAs (LCFAs), we first discovered a fungi/bacteria-originated polyketide synthase (PKS) system which involves a cytoplasmic acyl-ACP thioesterase (NoTE1). NoTE1 hydrolyzes C16:0-, C16:1- and C18:1-ACP in vitro and thus intercepts the specific acyl-ACPs elongated by PKS for polyunsaturated FA biosynthesis, resulting in elevation of C16/C18 monounsaturated FAs when overproduced and increase of C20 when knocked out. For medium-chain FAs (MCFAs; C8-C14), C8:0 and C10:0 FAs are boosted by introducing a Cuphea palustris acyl-ACP TE (CpTE), whereas C12:0 elevated by rationally engineering CpTE enzyme & rsquo;s substrate-binding pocket to shift its CL preference towards C12:0. A mechanistic model exploiting both native and engineered PKS and type II FAS pathways was thus proposed for manipulation of carbon distribution among FAs of various CL. The ability to tailor FA profile at the unit CL resolution from C8 to C20 in Nannochloropsis spp. lays the foundation for scalable production of designer lipids via industrial oleaginous microalgae. | - |
dc.identifier.bibliographicCitation | METABOLIC ENGINEERING, v.66, pp.157 - 166 | - |
dc.identifier.doi | 10.1016/j.ymben.2021.03.015 | - |
dc.identifier.issn | 1096-7176 | - |
dc.identifier.scopusid | 2-s2.0-85105698069 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/53128 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S1096717621000513?via%3Dihub | - |
dc.identifier.wosid | 000658808700004 | - |
dc.language | 영어 | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Manipulating fatty-acid profile at unit chain-length resolution in the model industrial oleaginous microalgae Nannochloropsis | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Biofuels | - |
dc.subject.keywordAuthor | Fatty acids | - |
dc.subject.keywordAuthor | Industrial microalgae | - |
dc.subject.keywordAuthor | Acyl-acyl carrier protein thioesterase | - |
dc.subject.keywordAuthor | Nannochloropsis | - |
dc.subject.keywordPlus | ACYL-ACP THIOESTERASE | - |
dc.subject.keywordPlus | DUNALIELLA-TERTIOLECTA | - |
dc.subject.keywordPlus | POLYKETIDE SYNTHASES | - |
dc.subject.keywordPlus | SYNTHETIC BIOLOGY | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | OIL | - |
dc.subject.keywordPlus | OCEANICA | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | SELECTIVITY | - |
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