Single Chamber Based Cell Concentration, DNA Extraction, and Real-Time PCR for Rapid Pathogen Identification

Abstract

Recently we reported a novel cell lysis method utilizing a 808 nm laser and magnetic beads[1]. Addition of magnetic particles to the cultured bacteria solutions accelerated the heat transfer rates. As a result, only 40 seconds of laser irradiation effectively lysed pathogens including both Gram-negative and Gram positive bacteria and hepatitis B viruses. In this report, we used magnetic micro particles modified with target specific antibodies and thus we could separate and concentrate target pathogens from whole blood and could do rapid and efficient DNA extraction using the same laser irradiation method. Furthermore, the same chip can be used in the real-time microchip based PCR machine, GenSpector TMC-1000[2] for the quantitative DNA analysis.