Amperometric Detection of Bisphenol-A on Laser Fabricated Capillary Electrophoresis Device

Abstract

In this study, poly dimethyl siloxane (PDMS) capillary or microchannel electrophoresis devices were fabricated and tested by laser writer. A programmable laser writer was controlled to make microchannel on PDMS mold instead of a conventional photolithography with SU-8. Indium tin oxide (ITO) electrodes for electrochemical detection of bisphenol-A were made by photolithography. UV-ozone treatment was used to bind laser patterned PDMS mold and patterned ITO glass. During electrophoresis, changes of current measured at working electrode were recorded with time by source measure unit. The peak current representing for detection of bisphenol-A was analyzed and compared with devices fabricated by laser and conventional lithography, respectively. Relationships among microchannel size, separation voltage, and peak currents were established.