Full metadata record
DC Field | Value | Language |
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dc.citation.startPage | 2041731421 | - |
dc.citation.title | JOURNAL OF TISSUE ENGINEERING | - |
dc.citation.volume | 12 | - |
dc.contributor.author | Jeong, Wonwoo | - |
dc.contributor.author | Kim, Min Kyeong | - |
dc.contributor.author | Kang, Hyun-Wook | - |
dc.date.accessioned | 2023-12-21T16:16:48Z | - |
dc.date.available | 2023-12-21T16:16:48Z | - |
dc.date.created | 2021-02-04 | - |
dc.date.issued | 2021-02 | - |
dc.description.abstract | Decellularized extracellular matrix-based bio-inks (dECM bio-inks) for bioprinting technology have recently gained attention owing to their excellent ability to confer tissue-specific functions and 3D-printing capability. Although decellularization has led to a major advancement in bio-ink development, the effects of detergent type, the most important factor in decellularization, are still unclear. In this study, the effects of various detergent types on bio-ink performance were investigated. Porcine liver-derived dECM bio-inks prepared using widely used detergents, including sodium dodecyl sulfate (SDS), sodium deoxycholate (SDC), Triton X-100 (TX), and TX with ammonium hydroxide (TXA), were characterized in detail. SDS and SDC severely damaged glycosaminoglycan and elastin proteins, TX showed the lowest rate of decellularization, and TXA-based dECM bio-ink possessed the highest ECM content among all bio-inks. Differences in biochemical composition directly affected bio-ink performance, with TXA-dECM bio-ink showing the best performance with respect to gelation kinetics, intermolecular bonding, mechanical properties, and 2D/3D printability. More importantly, cytocompatibility tests using primary mouse hepatocytes also showed that the TXA-dECM bio-ink improved albumin secretion and cytochrome P450 activity by approximately 2.12- and 1.67-fold, respectively, compared with the observed values for other bio-inks. Our results indicate that the detergent type has a great influence on dECM damage and that the higher the dECM content, the better the performance of the bio-ink for 3D bioprinting. | - |
dc.identifier.bibliographicCitation | JOURNAL OF TISSUE ENGINEERING, v.12, pp.2041731421 | - |
dc.identifier.doi | 10.1177/2041731421997091 | - |
dc.identifier.issn | 2041-7314 | - |
dc.identifier.scopusid | 2-s2.0-85101740878 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/49986 | - |
dc.identifier.url | https://journals.sagepub.com/doi/10.1177/2041731421997091 | - |
dc.identifier.wosid | 000624952400001 | - |
dc.language | 영어 | - |
dc.publisher | SAGE PUBLICATIONS INC | - |
dc.title | Effect of detergent type on the performance of liver decellularizedextracellular matrix-based bio-inks | - |
dc.type | Article | - |
dc.description.isOpenAccess | TRUE | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Bio-ink | - |
dc.subject.keywordAuthor | decellularization | - |
dc.subject.keywordAuthor | detergent | - |
dc.subject.keywordAuthor | 3D bioprinting | - |
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