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DC Field | Value | Language |
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dc.citation.startPage | 123194 | - |
dc.citation.title | BIORESOURCE TECHNOLOGY | - |
dc.citation.volume | 307 | - |
dc.contributor.author | Zhou, Shengfang | - |
dc.contributor.author | Lama, Suman | - |
dc.contributor.author | Jiang, Jihong | - |
dc.contributor.author | Sankaranarayanan, Mugesh | - |
dc.contributor.author | Park, Sunghoon | - |
dc.date.accessioned | 2023-12-21T17:16:49Z | - |
dc.date.available | 2023-12-21T17:16:49Z | - |
dc.date.created | 2020-05-19 | - |
dc.date.issued | 2020-07 | - |
dc.description.abstract | The use of acetate as carbon feedstock can enhance sustainability and economics of the current bio-productions. This study explored the potential of acetate for the production of 3-hydroxypropionic acid by engineered Pseudomonas denitrificans. Heterologous mcr (encoding malonyl-CoA reductase) from Chloroflexus aurantiacus and endogenous accABCD (encoding acetyl-CoA carboxylase) were overexpressed in P. denitrificans. Carbon flux to 3-HP synthesis at the malonyl-CoA node was promoted by suppressing fatty acid synthesis through addition of cerulenin or deletion of fabF gene. In addition, stimulation of glyoxylate shunt and/or TCA cycle were attempted. Recombinant P. denitrificans overexpressing mcr and accABCD produced 19.3?mM 3-HP with cerulenin addition, and 14.2 mM with fabF deletion, respectively. Furthermore, the non-growing cells devoid of fabF could continuously produce 3-HP up to 40.4 mM without losing its production activity for 22 h. This study demonstrates that acetate is a good substrate for 3-HP production by recombinant P. denitrificans. | - |
dc.identifier.bibliographicCitation | BIORESOURCE TECHNOLOGY, v.307, pp.123194 | - |
dc.identifier.doi | 10.1016/j.biortech.2020.123194 | - |
dc.identifier.issn | 0960-8524 | - |
dc.identifier.scopusid | 2-s2.0-85082338492 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/49125 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S096085242030465X | - |
dc.identifier.wosid | 000528857700064 | - |
dc.language | 영어 | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Use of acetate for the production of 3-hydroxypropionic acid by metabolically-engineered Pseudomonas denitrificans | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels | - |
dc.relation.journalResearchArea | Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | 3-Hydroxypropionic acid | - |
dc.subject.keywordAuthor | Acetate | - |
dc.subject.keywordAuthor | Pseudomonas denitrificans | - |
dc.subject.keywordAuthor | Acetyl-CoA | - |
dc.subject.keywordAuthor | Malonyl-CoA | - |
dc.subject.keywordPlus | MALONYL-COA PATHWAY | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | GLYOXYLATE SHUNT | - |
dc.subject.keywordPlus | FIXATION | - |
dc.subject.keywordPlus | CYCLE | - |
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