Full metadata record
DC Field | Value | Language |
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dc.citation.number | 1 | - |
dc.citation.startPage | 19064 | - |
dc.citation.title | SCIENTIFIC REPORTS | - |
dc.citation.volume | 10 | - |
dc.contributor.author | Thuan Phu Nguyen-Vo | - |
dc.contributor.author | Ko, Seyoung | - |
dc.contributor.author | Ryu, Huichang | - |
dc.contributor.author | Kim, Jung Rae | - |
dc.contributor.author | Kim, Donghyuk | - |
dc.contributor.author | Park, Sunghoon | - |
dc.date.accessioned | 2023-12-21T16:43:39Z | - |
dc.date.available | 2023-12-21T16:43:39Z | - |
dc.date.created | 2020-11-26 | - |
dc.date.issued | 2020-11 | - |
dc.description.abstract | Previously, we have reported that 3-hydroxypropionate (3-HP) tolerance in Escherichia coli W is improved by deletion of yieP, a less-studied transcription factor. Here, through systems analyses along with physiological and functional studies, we suggest that the yieP deletion improves 3-HP tolerance by upregulation of yohJK, encoding putative 3-HP transporter(s). The tolerance improvement by yieP deletion was highly specific to 3-HP, among various C2-C4 organic acids. Mapping of YieP binding sites (ChIP-exo) coupled with transcriptomic profiling (RNA-seq) advocated seven potential genes/operons for further functional analysis. Among them, the yohJK operon, encoding for novel transmembrane proteins, was the most responsible for the improved 3-HP tolerance; deletion of yohJK reduced 3-HP tolerance regardless of yieP deletion, and their subsequent complementation fully restored the tolerance in both the wild-type and yieP deletion mutant. When determined by 3-HP-responsive biosensor, a drastic reduction of intracellular 3-HP was observed upon yieP deletion or yohJK overexpression, suggesting that yohJK encodes for novel 3-HP exporter(s). | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, v.10, no.1, pp.19064 | - |
dc.identifier.doi | 10.1038/s41598-020-76120-3 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.scopusid | 2-s2.0-85094974499 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/48859 | - |
dc.identifier.url | https://www.nature.com/articles/s41598-020-76120-3 | - |
dc.identifier.wosid | 000587638900019 | - |
dc.language | 영어 | - |
dc.publisher | NATURE RESEARCH | - |
dc.title | Systems evaluation reveals novel transporter YohJK renders 3-hydroxypropionate tolerance in Escherichia coli | - |
dc.type | Article | - |
dc.description.isOpenAccess | TRUE | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | ACID STRESS | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | EVOLUTION | - |
dc.subject.keywordPlus | ACTIVATOR | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | GENOME | - |
dc.subject.keywordPlus | GENE | - |
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