File Download

  • Find it @ UNIST can give you direct access to the published full text of this article. (UNISTARs only)
Related Researcher

김하진

Kim, Hajin
Single Molecule Biophysics Lab.
Read More

Views & Downloads

Detailed Information

Cited time in webofscience Cited time in scopus
Metadata Downloads

The dynamic landscape of transcription initiation in yeast mitochondria

Author(s)
Sohn, Byeong-KwonBasu, UrmimalaLee, Seung-WonCho, HayoonShen, JiayuDeshpande, AishwaryaJohnson, Laura C.Das, KalyanPatel, Smita S.Kim, Hajin
Issued Date
2020-08
DOI
10.1038/s41467-020-17793-2
URI
https://scholarworks.unist.ac.kr/handle/201301/48338
Fulltext
https://www.nature.com/articles/s41467-020-17793-2
Citation
NATURE COMMUNICATIONS, v.11, no.1, pp.4281
Abstract
Controlling efficiency and fidelity in the early stage of mitochondrial DNA transcription is crucial for regulating cellular energy metabolism. Conformational transitions of the transcription initiation complex must be central for such control, but how the conformational dynamics progress throughout transcription initiation remains unknown. Here, we use single-molecule fluorescence resonance energy transfer techniques to examine the conformational dynamics of the transcriptional system of yeast mitochondria with single-base resolution. We show that the yeast mitochondrial transcriptional complex dynamically transitions among closed, open, and scrunched states throughout the initiation stage. Then abruptly at position +8, the dynamic states of initiation make a sharp irreversible transition to an unbent conformation with associated promoter release. Remarkably, stalled initiation complexes remain in dynamic scrunching and unscrunching states without dissociating the RNA transcript, implying the existence of backtracking transitions with possible regulatory roles. The dynamic landscape of transcription initiation suggests a kinetically driven regulation of mitochondrial transcription. Conformational dynamics during the early stage of transcription is crucial to understanding the regulation of transcription efficiency and fidelity. Here the authors, by single-molecule fluorescence resonance energy transfer approaches, examine the conformational dynamics of the two-component transcription system of yeast mitochondria with single-base resolution.
Publisher
NATURE PUBLISHING GROUP
ISSN
2041-1723
Keyword
CONFORMATIONAL HETEROGENEITYT7 RNA-POLYMERASEESCHERICHIA-COLIPROMOTER RECOGNITIONSPECIFICITY FACTORELONGATION-FACTORABORTIVE INITIATIONSTRUCTURAL BASISTEMPLATE STRANDIN-VITRO

qrcode

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.