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Sestrin2 interacts and regulates Orai1 calcium channels

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Title
Sestrin2 interacts and regulates Orai1 calcium channels
Author
Jang, Ki Hyun
Advisor
Park, Chan Young
Keywords
SOCE; STIM1; Orai1; SESN2
Issue Date
2020-08
Publisher
Graduate School of UNIST
Abstract
Store-operated Ca2+ entry (SOCE) is based on the interaction of endoplasmic reticulum (ER) Ca2+ sensor stromal interaction molecule 1 (STIM1) with the Orai1 Ca2+ channel which is located in plasma membrane. SOCE through Orai1 channels is induced by the store depletion in ER. Orai1 channels have unique regulation mechanism and its stable regulation is crucial for human health. To identify Orai1 activation and regulation mechanism further, I checked the proteins that bind with Orai1 through affinity prufication coupled with mass spectrometry (MS). I identified approximately 3,000 proteins which bind with Orai1. I noticed sestrin2 (SESN2) among those proteins. Stress inducible protein SESN2 response to several stresses like DNA damage, hypoxia and oxidative stress. SESN2 is known to regulate cell growth, metabolism. In this research, I investigated the interaction between Orai1 and SESN2. I found that SOCE activation transcriptionally up-regulates SESN2 mRNA expression. Additionally, I confirmed that Orai1 binds and co-localizes with SESN2. Finally, To elucidate the functional role of SESN2 in SOCE activation, I examined the effects of SESN2 on SOCE. I confirmed that SESN2 activates SOCE by using Fura-2AM Ca2+ imaging and NFAT luciferase assay. These results indicate that SESN2 interacts and regulates Orai1 calcium channels.
Description
Department of Biological Sciences
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