Developing an alternative model system of muco-ciliary epithelium and the study of the ERAD function on chondrogenesis
Cited 0 times inCited 0 times in
- Developing an alternative model system of muco-ciliary epithelium and the study of the ERAD function on chondrogenesis
- Sim, Hyo Jung
- Park. Tae Joo
- Issue Date
- Graduate School of UNIST
- Xenopus laevis is a well-known model animal to study developmental biology. Hundreds of eggs can be easily obtained from a single female by ovulation using human chorionic gonadotrophin (HCG) hormone, and developing embryos can be synchronized by in vitro fertilization. Also, Xenopus laevis egg is relatively larger than other model animals such as drosophila, zebrafish, and mouse. Furthermore, gene expression can be simply manipulated by performing mRNA or morpholino microinjection. Recently, CRISPR/Cas9 -mediated mutagenesis has been widely used to generate an F0 mutant using Xenopus embryos. Moreover, the developmental process is relatively faster compared with other animal models such as mouse or rats. Due to these advantages, I preferentially used Xenopus laevis as a model animal to conduct my graduate research project.
My graduate research has two main ideas. First, my research focuses on studying the pathophysiology of the muco-ciliary epithelium. Secondly, I researched craniofacial cartilage development. In muco-ciliary epithelium research, I revealed proteomes of ciliary subdomains, especially transition zone and ciliary axoneme. Also, I developed a high-throughput screening system to discover muco-active reagents. In craniofacial cartilage research, I demonstrated that the ERAD complex is critical for chondrogenesis and is one of the significant causes of osteoarthritis.
- Department of Biological Sciences
- Go to Link;
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
can give you direct access to the published full text of this article. (UNISTARs only)
Show full item record
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.