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Kwon, Hyug Moo
Immunometabolism and Cancer Lab
Research Interests
  • TonEBP, Obesity, Cancer, Chronic inflammatory diseases, Brain disorder, Kidney disorder, Genomic instability


Cis- and trans-acting factors regulating transcription of the BGT1 gene in response to hypertonicity

DC Field Value Language Miyakawa, H ko Woo, SK ko Chen, CP ko Dahl, SC ko Handler, JS ko Kwon, H. Moo ko 2014-05-22T05:02:12Z - 2014-05-21 ko 1998-04 ko
dc.identifier.citation AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, v.274, no.4, pp.f753 - f761 ko
dc.identifier.issn 1931-857X ko
dc.identifier.uri -
dc.description.abstract We have previously identified a tonicity-responsive enhancer (TonE) in the promoter region of the canine BGT1 gene. Tone mediates hypertonicity- induced stimulation of transcription. Here, we characterize Tone and Tone binding proteins (TonEBPs) to provide a biochemical basis for cloning of the TOnEBPs. Mutational analysis applied to both hypertonicity-induced stimulation of transcription and TonEBP binding reveals that Tone is 11 base pairs in length, with the consensus sequence of (C/T)GGAAnnn(C/T)n(C/T). Activity of the TonEBPs increases in response to hypertonicity with a time course similar to that of transcription of the BGT1 gene. Studies with inhibitors indicate that translation, but not transcription, is required for activation of the TonEBPs. Phosphorylation is required for the stimulation of transcription but not for activation of DNA binding by the TonEBPs. In vivo methylation by dimethyl sulfate reveals that the Tone site of the BGT1 gene is protected with a time course like that of activity of the TonEBPs and activation of transcription. Ultraviolet cross-linking indicates that the TonEBPs share a DNA binding subunit of 200 kDa. ko
dc.description.statementofresponsibility close -
dc.language 영어 ko
dc.title Cis- and trans-acting factors regulating transcription of the BGT1 gene in response to hypertonicity ko
dc.type ARTICLE ko
dc.identifier.scopusid 2-s2.0-0031958591 ko
dc.identifier.wosid 000073011000016 ko
dc.type.rims ART ko
dc.description.wostc 103 *
dc.description.scopustc 107 * 2014-10-18 * 2014-07-12 *
dc.identifier.url ko
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