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DC Field | Value | Language |
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dc.citation.endPage | 498 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 489 | - |
dc.citation.title | ANALYTICAL AND BIOANALYTICAL CHEMISTRY | - |
dc.citation.volume | 398 | - |
dc.contributor.author | Osiri, John K. | - |
dc.contributor.author | Shadpour, Hamed | - |
dc.contributor.author | Soper, Steven A. | - |
dc.date.accessioned | 2023-12-22T07:06:10Z | - |
dc.date.available | 2023-12-22T07:06:10Z | - |
dc.date.created | 2013-06-04 | - |
dc.date.issued | 2010-09 | - |
dc.description.abstract | A poly(methyl methacrylate) microfluidic chip was used to perform a two-dimensional (2-D) separation of a complex protein mixture in short development times. The separation was performed by combining sodium dodecyl sulfate micro-capillary gel electrophoresis (SDS mu-CGE) with microemulsion electrokinetic chromatography (mu-MEEKC), which were used for the first and second dimensions, respectively. Fluorescently labeled Escherichia coli cytosolic proteins were profiled by this 2-D approach with the results compared to a similar 2-D separation using SDS mu-CGE x mu-MEKC (micelle electrokinetic chromatography). The relatively short column lengths (effective length = 10 mm) for both dimensions were used to achieve separations requiring only 220 s of development time. High spot production rates (131 +/- 11 spots min(-1)) and reasonable peak capacities (481 +/- 18) were generated despite the fact that short columns were used. In addition, the use of mu-MEEKC in the second dimension was found to produce higher peak capacities compared to mu-MEKC (481 +/- 18 for mu-MEEKC and 332 +/- 17 for mu-MEKC) due to the higher plate numbers associated with mu-MEEKC. | - |
dc.identifier.bibliographicCitation | ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v.398, no.1, pp.489 - 498 | - |
dc.identifier.doi | 10.1007/s00216-010-3914-2 | - |
dc.identifier.issn | 1618-2642 | - |
dc.identifier.scopusid | 2-s2.0-77955980315 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/4612 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=77955980315 | - |
dc.identifier.wosid | 000280807500040 | - |
dc.language | 영어 | - |
dc.publisher | SPRINGER HEIDELBERG | - |
dc.title | Ultra-fast two-dimensional microchip electrophoresis using SDS mu-CGE and microemulsion electrokinetic chromatography for protein separations | - |
dc.type | Article | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods; Chemistry, Analytical | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology; Chemistry | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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