dc.citation.conferencePlace |
US |
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dc.citation.conferencePlace |
Boston; USA |
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dc.citation.title |
American Society for Biochemistry and Molecular Biology 2000 Annual Meeting |
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dc.contributor.author |
Hwang, J.-I. |
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dc.contributor.author |
Heo, K. |
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dc.contributor.author |
Shin, K.-J. |
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dc.contributor.author |
Kim, E. |
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dc.contributor.author |
Yun, C.-H.C. |
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dc.contributor.author |
Ryu, S.H. |
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dc.contributor.author |
Shin, H.-S. |
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dc.contributor.author |
Suh, Pann-Ghill |
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dc.date.accessioned |
2023-12-20T06:36:55Z |
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dc.date.available |
2023-12-20T06:36:55Z |
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dc.date.created |
2014-12-23 |
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dc.date.issued |
2000-06-04 |
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dc.description.abstract |
Among the phospholipase C that catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate, four mammalian phospholipase C-β (PLC-β) isotypes (isotypes 1-4) are activated through G protein-coupled receptors (GPCRs). Although the regulation of the PLC-βs by GPCRs and heterotrimeric G proteins has been extensively studied, little is known about the molecular determinants that regulate their activity. The PLC-β isozymes carry a putative PSD-95/Dlg/ZO-1 (PDZ) binding motif (X(S/T)X(V/L)COOH) at their carboxyl terminus, which is implicated in specific interactions with anchor proteins. Using the yeast two-hybrid system, we identified Na+/H+ exchanger regulatory factor 2 (NHERF2) as a protein that interacted with a C- terminal heptapeptide of PLC-β3. Immunoprecipitation studies revealed that NHERF2 interacts specifically with PLCβ3, but not with other PLC-β isotypes. Furthermore, PLC-β3 interacted with NHERF2 rather than with other PDZ-containing proteins. This interaction required the COOH-terminal NTQL sequence of PLC-β3 and the second PDZ domain of NHERF2. Interestingly, NHERF2 potentiated the PLC-β activation by carbachol in COS7 and HeLa cells, while mutant NHERF2, lacking the second PDZ domain, had no such effect. Taken together, the data suggest that NHERF2 may act as a modulator underlying the process of PLC-β3-mediated signaling. |
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dc.identifier.bibliographicCitation |
American Society for Biochemistry and Molecular Biology 2000 Annual Meeting |
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dc.identifier.uri |
https://scholarworks.unist.ac.kr/handle/201301/45125 |
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dc.publisher |
American Society for Biochemistry and Molecular Biology |
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dc.title |
Regulation of phospholipase Cβ3 activity by Na+/H+ exchanger regulatory factor 2 |
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dc.type |
Conference Paper |
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dc.date.conferenceDate |
2000-06-04 |
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